CADMIUM-SENSITIVE, CAD1 MUTANTS OF ARABIDOPSIS-THALIANA ARE PHYTOCHELATIN DEFICIENT
CADMIUM-SENSITIVE, CAD1 MUTANTS OF ARABIDOPSIS-THALIANA ARE PHYTOCHELATIN DEFICIENT
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DOI：
10.1104/pp.107.4.1059
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发表时间：
1995-04-01
期刊：
影响因子：
7.4
通讯作者：
COBBETT, CS
中科院分区：
文献类型：
--
作者：
HOWDEN, R;GOLDSBROUGH, PB;COBBETT, CS
从拟南芥中分离出镉敏感突变体cad1等位基因系列。这些突变体对镉有不同程度的敏感性，凝胶过滤色谱检测发现它们缺乏形成镉肽复合物的能力。通过高效液相色谱法检测，每个突变体都缺乏积累植物螯合素（PCs）的能力，并且每个突变体积累PCs的数量与其对镉的敏感程度相关。这些突变体具有野生型谷胱甘肽水平，谷胱甘肽是PC生物合成的底物，体外实验表明，每个突变体都缺乏PC合成酶活性。这些结果有力地证明了pc对植物耐镉性的重要性。
An allelic series of cad1, cadmium-sensitive mutants of Arabidopsis thaliana, was isolated. These mutants were sensitive to cadmium to different extents and were deficient in their ability to form cadmium-peptide complexes as detected by gel-filtration chromatography. Each mutant was deficient in its ability to accumulate phytochelatins (PCs) as detected by high-performance liquid chromatography and the amount of PCs accumulated by each mutant correlated with ifs degree of sensitivity to cadmium. The mutants had wild-type levels of glutathione, the substrate for PC biosynthesis, and in vitro assays demonstrated that each of the mutants was deficient in PC synthase activity. These results demonstrate conclusively the importance of PCs for cadmium tolerance in plants.