Cellular uptake, stability, visualization by 'Naturstoff reagent A', and multidrug resistance protein 1 gene-regulatory activity of cyanidin in human keratinocytes
Cellular uptake, stability, visualization by 'Naturstoff reagent A', and multidrug resistance protein 1 gene-regulatory activity of cyanidin in human keratinocytes
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DOI：
10.1016/j.phrs.2009.10.006
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发表时间：
2010-03-01
影响因子：
9.3
通讯作者：
Rimbach, G.
中科院分区：
文献类型：
--
作者：
Ernst, I. M. A.;Wagner, A. E.;Rimbach, G.
人们对花青素作为潜在的皮肤保护植物化学物质的作用越来越感兴趣。然而，很少有人知道花青素是否被人体皮肤吸收，以及在多大程度上被人体皮肤吸收。本研究在培养的人HaCaT角质形成细胞中测定了花青素的细胞摄取（HPLC测定）、稳定性和基因调控活性。采用荧光染料Naturstoff试剂A对花青素进行可视化观察，通过流式细胞术和荧光显微镜观察其细胞积累情况。较低浓度的花青素被HaCaT细胞迅速吸收。孵育后，细胞花青素水平随时间下降，很可能是由于降解为原儿茶酸和间苯三酚醛。共聚焦激光扫描显微镜数据显示，花青素主要存在于细胞质中。细胞对花青素的摄取伴随着多药耐药蛋白1（参与黄酮类化合物的细胞外排）mrna水平的抑制，表明其基因调控活性。Naturstoff试剂A似乎是一种很有前途的荧光染料，用于可视化角质形成细胞中的花青素。2009爱思唯尔有限公司版权所有。
There is increasing interest in the role of anthocyanidins as potential skin protective phytochemicals. However, little is known if and to what extent anthocyanidins are taken up by the human skin. In the present study cellular uptake (as determined by HPLC), stability, and gene-regulatory activity of cyanidin were determined in human HaCaT keratinocytes in culture. Using the fluorescent dye Naturstoff reagent A cyanidin was visualized in order to determine its cellular accumulation via flow cytometry and fluorescence microscopy.Cyanidin was rapidly taken up by HaCaT cells at relatively low concentrations. Following incubation, cellular cyanidin levels decreased time-dependently most likely due to degradation into protocatechuic acid and phloroglucinol aldehyde. Confocal laser scanning microscopy data demonstrated that cyanidin was mainly present in the cytoplasm. Cellular uptake of cyanidin was accompanied by an inhibition of multidrug resistance protein 1 (involved in cellular efflux of flavonoids) mRNA-levels indicating its gene-regulatory activity.Naturstoff reagent A seems to be a promising fluorescent dye to visualize cyanidin in keratinocytes. (C) 2009 Elsevier Ltd. All rights reserved.