Phenotypic and Functional Alterations on Inflammatory Peripheral Blood Cells After Acute Myocardial Infarction
Phenotypic and Functional Alterations on Inflammatory Peripheral Blood Cells After Acute Myocardial Infarction
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DOI：
10.1007/s12265-012-9365-8
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发表时间：
2012-06-01
影响因子：
3.4
通讯作者：
Paiva, Artur
中科院分区：
文献类型：
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作者：
Carvalheiro, Tiago;Velada, Isabel;Paiva, Artur
对12例急性心肌梗死(AMI)患者(T0)、发病1个月(T1)和12例健康人(HG)T细胞、单核细胞和树突状细胞亚群的频率和功能进行了研究。细胞分选后，用流式细胞仪和RT-PCR进行细胞特性和功能研究。在T0时刻，与T1和HG相比，最重要的发现是：产生IL-2的T细胞的频率降低；产生TNF-α和IL-6的单核细胞、髓系树突状细胞和CD14(-/低)CD16(+)树突状细胞的频率降低；分离这些细胞后，TNF-α的mRNA表达降低。此外，随着FoxP3、CTLA-4和TGF-βmRNA表达的增加，Treg细胞的调节功能在T0时刻上调。这些表型和功能改变中的大多数在T1时消失。我们的数据表明，急性心肌梗死导致免疫系统动态平衡的显著变化。
The frequency and function of T cells, monocytes, and dendritic cell subsets were investigated in 12 patients after acute myocardial infarction (AMI)-(T0), 1 month after the episode (T1), and in 12 healthy individuals (HG). The cell characterization and the functional studies were performed by flow cytometry and by RT-PCR, after cell sorting. The most important findings at T0 moment, when compared with T1 and HG, were: a decrease in the frequency of IL-2-producing T cells; a lower frequency of TNF-alpha- and IL-6-producing monocytes, myeloid dendritic cells, and CD14(-/low)CD16(+)DCs; and a lower TNF-alpha mRNA expression, after sorting these cells. Moreover, the regulatory function of Treg cells, at T0 moment, was upregulated, based on the FoxP3, CTLA-4, and TGF-beta mRNA expression increase. The majority of these phenotypic and functional alterations disappeared at T1. Our data demonstrate that AMI induces a significant change in the immune system homeostasis.