Genomic changes during chronic Helicobacter pylori infection
Genomic changes during chronic Helicobacter pylori infection
复制标题
DOI：
10.1128/jb.188.1.249-254.2006
复制
发表时间：
2006-01-01
影响因子：
3.2
通讯作者：
Suerbaum, S
中科院分区：
文献类型：
--
作者：
Kraft, C;Stack, A;Suerbaum, S
胃病原体幽门螺杆菌在人群中表现出巨大的遗传变异，无论是在基因内容还是在序列水平上。我们通过比较从同一患者不同时间点采集的 21 对密切相关的分离株的基因组含量，研究了这种变异性是如何产生的。通过与全基因组 DNA 微阵列杂交进行比较。对微阵列表明基因组变化的所有位点进行测序以确认事件。将基因组变化的数量与我们之前通过多位点序列分析和基于序列数据的数学建模确定的不丢失或获得基因的同源替换事件的数量进行比较。我们的分析表明，绝大多数遗传变化是由于同源重组，其中 1/650 事件导致基因的净增加或损失。这些结果表明，幽门螺杆菌对宿主个体的适应可能主要是通过序列变化而不是基因的丢失或获得而发生的。
The gastric pathogen Helicobacter pylori shows tremendous genetic variability within human populations, both in gene content and at the sequence level. We investigated how this variability arises by comparing the genome content of 21 closely related pairs of isolates taken from the same patient at different time points. The comparisons were performed by hybridization with whole-genome DNA microarrays. All loci where microarrays indicated a genomic change were sequenced to confirm the events. The number of genomic changes was compared to the number of homologous replacement events without loss or gain of genes that we had previously determined by multilocus sequence analysis and mathematical modeling based on the sequence data. Our analysis showed that the great majority of genetic changes were due to homologous recombination, with 1/650 events leading to a net gain or loss of genes. These results suggest that adaptation of H. pylori to the host individual may principally occur through sequence changes rather than loss or gain of genes.