DELETIONS IN THE 3'-TERMINAL TRANSFER RNA-LIKE STRUCTURE OF BROME MOSAIC-VIRUS RNA DIFFERENTIALLY AFFECT AMINOACYLATION AND REPLICATION INVITRO
DELETIONS IN THE 3'-TERMINAL TRANSFER RNA-LIKE STRUCTURE OF BROME MOSAIC-VIRUS RNA DIFFERENTIALLY AFFECT AMINOACYLATION AND REPLICATION INVITRO
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DOI：
10.1073/pnas.82.17.5636
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
HALL, TC
中科院分区：
文献类型：
--
作者：
BUJARSKI, JJ;DREHER, TW;HALL, TC
通过S1核酸酶处理在几个不同限制性位点线性化的克隆DNA，产生覆盖雀麦花叶病毒RNA 3的3“201个核苷酸的cDNA克隆的缺失。这些克隆的转录产生了在3“-末端tRNA样结构中含有结构改变的RNA，其参与氨酰化和复制。复制酶模板活性，但不是氨酰化活性，是特别敏感的臂C，其中包含一个酪氨酰反密码子的缺失。臂B中的缺失对氨酰化是有害的，但是复制酶模板活性损失的比例取决于缺失的位点。臂D的去除对氨酰化几乎没有影响，并且在某些情况下，导致重复模板活性的2倍刺激。
Deletions in cDNA clones covering the 3'' 201 nucleotides of brome mosaic virus RNA 3 were produced by S1 nuclease treatment of cloned DNA linearized at several different restriction sites. Transcription of these clones yielded RNAs containing structural alterations in the 3''-terminal tRNA-like structure that is involved in aminoacylation and replication. Replicase template activity, but not aminoacylation activity, was especially sensitive to deletions in arm C, which contains a tyrosyl anticodon. Deletions in arm B were detrimental to aminoacylation, bu the proportion of replicase template activity lost depended on the site of deletion. Removal of arm D had little effect on aminoacylation and, in some instances, resulted in a 2-fold stimulation of replicate template activity.