Recruitment and Phenotypic Characteristics of Interleukin 9-Producing CD4+ T Cells in Malignant Pleural Effusion
Recruitment and Phenotypic Characteristics of Interleukin 9-Producing CD4+ T Cells in Malignant Pleural Effusion
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DOI：
10.1007/s00408-013-9474-4
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发表时间：
2013-08-01
期刊：
影响因子：
5
通讯作者：
Shi, Huan-Zhong
中科院分区：
文献类型：
--
作者：
Bu, Xiao-Ning;Zhou, Qiong;Shi, Huan-Zhong
我们之前的数据表明，恶性胸腔积液（MPE）中产生IL-9的CD4（+）T细胞（Th9细胞）的数量与血液中相比显着增加。本研究旨在探讨Th9细胞募集到MPE的机制以及胸膜Th9细胞的表型特征。观察Th9细胞上趋化因子受体（CCR）的表达模式以及趋化因子CCL20对Th9细胞的体外趋化活性。通过流式细胞术测定MPE中Th9细胞的表型特征。我们发现MPE和血液中的Th9细胞表面均表达高水平的CCR6。体外迁移实验证实，MPE和培养的胸膜间皮细胞上清液均可诱导Th9细胞迁移，抗CCL20 mAb显着抑制MPE或上清液刺激Th9细胞趋化的能力。我们还注意到，胸膜 Th9 细胞表达高水平的 CD45RO 和极低水平的 CD45RA 和 CD62L，显示出效应记忆细胞的表型。我们的数据显示，胸膜 CCL20 可以诱导 Th9 细胞募集到 MPE 中，并且 MPE 中的大多数 Th9 细胞显示出效应记忆细胞的表型。
Our previous data have demonstrated that the number of IL-9-producing CD4(+) T cells (Th9 cells) in malignant pleural effusion (MPE) was significantly increased when compared with that in blood. The aim of the present study was to investigate the mechanism by which Th9 cells were recruited into MPE and the phenotypic characteristics of pleural Th9 cells.The expression patterns of chemokine receptors (CCRs) on Th9 cells and the chemoattractant activity of chemokine CCL20 for Th9 cells in vitro were observed. The phenotypic features of Th9 cells in MPE were determined by flow cytometry.We found that Th9 cells in both MPE and blood expressed a high level of CCR6 on their surface. An in vitro migration assay confirmed that both MPE and supernatants of cultured pleural mesothelial cells could induce the migration of Th9 cells, and anti-CCL20 mAb significantly inhibited the ability of MPE or supernatants to stimulate Th9 cell chemotaxis. We also noted that pleural Th9 cells expressed high levels of CD45RO and very low levels of CD45RA and CD62L, displaying the phenotype of effector memory cells.Our data revealed that recruitment of Th9 cells into MPE could be induced by pleural CCL20 and that the majority of Th9 cells in MPE displayed the phenotype of effector memory cells.