Methods for Single Cell Transcriptomic Analysis of Adipose Tissue.
Methods for Single Cell Transcriptomic Analysis of Adipose Tissue.
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脂肪组织的单细胞转录组分析方法。
DOI：
10.1007/978-1-0716-3167-6_21
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Shamsi,Farnaz
中科院分区：
文献类型：
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作者：
Shamsi,Farnaz
单细胞方法的发展促进了复杂组织中细胞异质性和细胞类型特异性基因表达的研究。脂肪组织库包含储存脂肪的脂肪细胞，以及形成脂肪细胞生态位并调节脂肪组织功能的各种细胞类型。在这里，我描述了从白色和棕色脂肪组织中分离单个细胞和核的两种方案。此外，我还提供了使用核标记和翻译核糖体亲和纯化(NuTRAP)小鼠模型来分离特定细胞类型或谱系的单核的详细工作流程。
The development of single cell approaches has facilitated the investigation of cellular heterogeneity and cell type-specific gene expression in complex tissues. Adipose tissue depots contain lipid storing adipocytes as well as a diverse array of cell types that form the adipocyte niche and regulate adipose tissue function. Here, I describe two protocols for the isolation of single cells and nuclei from white and brown adipose tissue. Additionally, I provide a detailed workflow for isolation of cell type- or lineage-specific single nuclei using nuclear tagging and translating ribosome affinity purification (NuTRAP) mouse models.