OCCURRENCE AND DEVELOPMENTAL PATTERN OF NEUROMEDIN U-IMMUNOREACTIVE NERVES IN THE GASTROINTESTINAL-TRACT AND BRAIN OF THE RAT
OCCURRENCE AND DEVELOPMENTAL PATTERN OF NEUROMEDIN U-IMMUNOREACTIVE NERVES IN THE GASTROINTESTINAL-TRACT AND BRAIN OF THE RAT
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DOI：
10.1016/0306-4522(88)90037-1
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发表时间：
1988-06-01
期刊：
影响因子：
3.3
通讯作者：
POLAK, JM
中科院分区：
文献类型：
--
作者：
BALLESTA, J;CARLEI, F;POLAK, JM
Neuromedin U是一种新发现的调节肽，通过放射免疫分析发现在大鼠的脑和肠中均存在显著浓度。本研究的目的是本地化这种肽的免疫反应，肠和脑的离散结构，并利用免疫细胞化学绘制其分布。在肠道中，神经介肽U仅限于主要在肌间神经丛和粘膜下神经丛以及除胃以外的所有区域的粘膜中的神经纤维。免疫反应性神经节细胞被认为是在这两个神经节丛和他们的数量并没有增加秋水仙碱管理。这一观察结果和回肠中神经介肽U免疫反应性神经的群体不受完全的外源性去神经支配的影响的发现表明，神经主要是内在起源的。共定位研究表明，神经介肽U和降钙素基因相关肽存在于相同的肌间和粘膜下神经节细胞。横切实验表明，像降钙素基因相关肽免疫反应神经，纤维含有神经介肽U项目非常短的距离在口腔和肛门的方向。在电子显微镜水平上，神经介肽U免疫反应，证明使用免疫金技术，被定位到大颗粒囊泡。在中枢神经系统中，神经介肽U免疫反应性被定位到纤维，广泛分布在整个大脑，除了在小脑。神经介肽U免疫反应阳性细胞体的存在仅限于弓状核的吻尾侧部分。共定位研究表明，弓状核中的神经介肽U免疫反应阳性细胞体的比例也包含前阿黑皮素。神经介肽U-免疫反应纤维首次检测到大鼠肠粘膜在出生后第1天。在大脑中，弓状核显示神经介肽U免疫反应神经元胞体在E16，但不是在E14。总之，神经介肽U是脑肠肽分子组的新成员。
Neuromedin U is a newly described regulatory peptide, found by radioimmunoassay in significant concentrations in both the brain and gut of the rat. The aim of the present study was to localize this peptide immunoreactivity to discrete structures of the gut and brain and to map its distribution using immunocytochemistry. In the gut, neuromedin U was confined to nerve fibres mainly in the myenteric and submucous plexuses and the mucosa of all areas except stomach. Immunoreactive ganglion cells were seen in both ganglionated plexuses and their number did not increase following colchicine administration. This observation and the finding that the population of neuromedin U-immunoreactive nerves in the ileum was not affected by complete extrinsic denervation indicated that the nerves are mostly intrinsic in origin. Colocalization studies revealed neuromedin U and calcitonin gene-related peptide were present in the same myenteric and submucosal ganglion cells. Transection experiments showed that, like calcitonin gene-related peptide-immunoreactive nerves, fibres containing neuromedin U project for very short distances in both an oral and anal direction. At the electron microscopic level, neuromedin U immunoreactivity, demonstrated using the immunogold technique, was localized to large granular vesicles. In the central nervous system, neuromedin U immunoreactivity was localized to fibres which were widespread throughout the brain, except in the cerebellum. The presence of neuromedin U-immunoreactive cell bodies was restricted to the rostrocaudal part of the arcuate nucleus. Colocalization studies showed that a proportion of the neuromedin U-immunoreactive cell bodies in the arcuate nucleus also contained pro-opiomelanocortin. Neuromedin U-immunoreactive fibres were first detected in the rat intestinal mucosa at day 1 after birth. In the brain, the arcuate nucleus showed neuromedin U-immunoreactive neuronal cell bodies at E16 but not at E14. In conclusion, neuromedin U is a new member of the group of molecules known as brain-gut peptides.