Enhancements in travelling wave ion mobility resolution
Enhancements in travelling wave ion mobility resolution
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DOI：
10.1002/rcm.5013
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发表时间：
2011-06-15
影响因子：
2
通讯作者：
Campuzano, Iain
中科院分区：
文献类型：
--
作者：
Giles, Kevin;Williams, Jonathan P.;Campuzano, Iain
使用离子迁移率分离来确定气相离子的碰撞截面可以提供有价值的结构信息。在四极杆/飞行时间质谱仪中引入行波离子迁移率，使得可以对在生理相关浓度下不同气相尺寸/结构和相对分子质量的一系列离子物种进行常规碰撞截面测量。在这里，我们讨论了第二代行波离子迁移率分离器的技术进步，其结果是迁移率分辨率提高了四倍。这一改善是通过两个反转肽(相对分子质量490 Da)、小分子含Ru抗癌药物(相对分子质量427 Da)、顺铂修饰蛋白(相对分子质量8776 Da)和非共价十四聚体伴侣络合物GroEL(相对分子质量802 kDa)证明的。结果还表明，用第二代迁移率分离器测定的碰撞截面与上一代和理论推导的值有很好的相关性。版权所有(C)2011 John Wiley&Sons，Ltd.
The use of ion mobility separation to determine the collision cross-section of a gas-phase ion can provide valuable structural information. The introduction of travelling-wave ion mobility within a quadrupole/time-of-flight mass spectrometer has afforded routine collision cross-section measurements to be performed on a range of ionic species differing in gas-phase size/structure and molecular weight at physiologically relevant concentrations. Herein we discuss the technical advances in the second-generation travelling-wave ion mobility separator, which result in up to a four-fold increase in mobility resolution. This improvement is demonstrated using two reverse peptides (mw 490 Da), small ruthenium-containing anticancer drugs (mw 427 Da), a cisplatin-modified protein (mw 8776 Da) and the noncovalent tetradecameric chaperone complex GroEL (mw 802 kDa). What is also shown are that the collision cross-sections determined using the second-generation mobility separator correlate well with the previous generation and theoretically derived values. Copyright (C) 2011 John Wiley & Sons, Ltd.