Plasma-membrane H+-ATPase gene expression is regulated by NaCl in cells of the halophyte Atriplex nummularia L.
Plasma-membrane H+-ATPase gene expression is regulated by NaCl in cells of the halophyte Atriplex nummularia L.
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DOI：
10.1007/bf00224780
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发表时间：
2004
期刊：
影响因子：
4.3
通讯作者：
X. Niu;Jian‐Kang Zhu;M. Narasimhan;R. Bressan;P. Hasegawa
中科院分区：
文献类型：
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作者：
X. Niu;Jian‐Kang Zhu;M. Narasimhan;R. Bressan;P. Hasegawa
AnAtriplex nummulariaL.分离了编码质膜H+-ATP酶亚型的部分序列的cDNA探针，并用于表征该盐生植物培养细胞中mRNA积累的NaCl调节。从开放阅读框翻译的肽（447 个氨基酸）与白花烟草质膜 H+-ATP 酶亚型 pma4 具有最高的序列同源性（大于 80% 同一性），并在总 RNA 和 Poly(A)+RNA 的 Northern 印迹上检测到约 3.7 kb 的转录物。未适应细胞、适应细胞（适应并在 342 mM NaCl 中生长的细胞）和去适应细胞（之前适应 342 mM NaCl 现在在无盐条件下生长的细胞）中的 mRNA 水平相当。暴露于 NaCl 后 24 小时内，在死适应细胞中检测到 mRNA 丰度增加，但在经过类似盐处理的未适应细胞中未检测到 mRNA 丰度增加。死适应细胞中 NaCl 信息丰度的上调受到发育控制。与糖植物的报道类似，质膜 H+-ATP 酶由盐生植物中的多基因家族编码。
AnAtriplex nummulariaL. cDNA probe encoding the partial sequence of an isoform of the plasma-membrane H+-ATPase was isolated, and used to characterize the NaCl regulation of mRNA accumulation in cultured cells of this halophyte. The peptide (447 amino acids) translated from the open reading frame has the highest sequence homology to theNicotiana plumbaginifoliaplasma-membrane H+-ATPase isoform pma4 (greater than 80% identity) and detected a transcript of approximately 3.7 kb on Northern blots of both total and poly(A)+RNA. The mRNA levels were comparable in unadapted cells, adapted cells (cells adapted to and growing in 342 mM NaCl) and deadapted cells (cells previously adapted to 342 mM NaCl that are now growing without salt). Increased mRNA abundance was detected in deadapted cells within 24 h after exposure to NaCl but not in unadapted cells with similar salt treatments. The NaCl up-regulation of message abundance in deadapted cells was subject to developmental control. Analogous to those reported for glycophytes, the plasma-membrane H+-ATPase are encoded by a multigene family in the halophyte.