INTERACTION OF A CYCLIC PEPTIDE, RO09-0198, WITH PHOSPHATIDYLETHANOLAMINE IN LIPOSOMAL MEMBRANES
INTERACTION OF A CYCLIC PEPTIDE, RO09-0198, WITH PHOSPHATIDYLETHANOLAMINE IN LIPOSOMAL MEMBRANES
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DOI：
10.1016/0005-2736(88)90193-9
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发表时间：
1988-05-24
期刊：
影响因子：
--
通讯作者：
INOUE, K
中科院分区：
文献类型：
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作者：
CHOUNG, SY;KOBAYASHI, T;INOUE, K
Ro 09 -0198是从灰轮枝链霉菌（Streptoveticillium griseoverticillatum）中分离的环肽。该肽引起含磷脂酰乙醇胺的脂质体的渗透性增加和聚集。然而，含有磷脂酰丝氨酸、磷脂酰肌醇或心磷脂而不是磷脂酰乙醇胺的脂质体与肽没有明显的反应性。在磷脂酰乙醇胺的结构类似物中，掺入脂质体中的二烷基磷脂酰乙醇胺和1-酰基甘油磷酸乙醇胺可与Ro 09 -0198相互作用，导致渗透性增加，而由烷基磷酸乙醇胺或磷脂酰-N-单甲基乙醇胺组成的脂质体对肽不敏感。这些结果表明，甘油骨架和磷脂酰乙醇胺的伯氨基是与Ro 09 -0198相互作用引起膜损伤所必需的，Ro 09 -0198诱导脂质体的选择性渗透性变化。葡萄糖和伞形酮磷酸酯被显著地外排，而蔗糖只有轻微的渗透性，菊粉不能被释放。因此，Ro 09 -0198诱导的渗透性增加对比蔗糖小的分子具有相当特异性。用Ro 09 -0198处理脂质体后，观察到自旋标记磷脂酰乙醇胺的电子自旋共振信号的谱线增宽。这些结果表明，Ro 09 -0198可以改变膜中磷脂酰乙醇胺的物理组织，从而为膜渗透性的变化提供了依据。
Ro09-0198 is a cyclic peptide isolated from Streptoverticillium griseoverticillatum. This peptide caused permeability increase and aggregation of liposomes containing phosphatidylethanolamine. Liposomes containing phosphatidylserine, phosphatidylinositol or cardiolipin instead of phosphatidylethanolamine were, however, not appreciably reactive with the peptide. Among the structural analogs of phosphatidylethanolamine, dialkylphosphatidylethanolamine and 1-acylglycerophosphoethanolamine incorporated into liposomes could interact with Ro09-0198 to cause a permeability increase, whereas liposomes consisting of alkylphosphoethanolamine or phosphatidyl-N-monomethylethanolamine were insensitive to the peptide. These findings indicate that a glycerol backbone and a primary amino group of phosphatidylethanolamine are necessary for interaction with Ro09-0198 to cause membrane damage, Ro09-0198 induced a selective permeability change on liposomes. Glucose and umbelliferyl phosphate were effluxed significantly, but sucrose was only slightly permeable and inulin could not be released. Consequently, the permeability increase induced by Ro09-0198 is rather specifc to molecules smaller than sucrose. Line broadening of electron spin resonance signals of spin-labeled phosphatidylethanolamine was observed upon treatment of liposomes with Ro09-0198. It was suggested from these results that Ro09-0198 can alter the physical organization of phosphatidylethanolamine in membranes, thus providing a basis for changes in membrane permeability.