Production of a copolyester of 3-hydroxybutyric acid and 3-hydroxyvaleric acid from single unrelated carbon sources by a mutant of Alcaligenes eutrophus
Production of a copolyester of 3-hydroxybutyric acid and 3-hydroxyvaleric acid from single unrelated carbon sources by a mutant of Alcaligenes eutrophus
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DOI：
10.1007/bf00174193
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发表时间：
1992-04
影响因子：
5
通讯作者：
A. Steinbüchel;U. Pieper
中科院分区：
文献类型：
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作者：
A. Steinbüchel;U. Pieper
Alcaligenes富营养化突变菌株R3是野生型菌株H16的异色氨酸营养缺陷突变体的原生营养的自发逆转，在培养基中生长必需的营养物质耗尽后，从各种单一不相关的底物中积累了由3-羟基丁酸（3HB）为主要成分和3-羟基戊酸（3HV），即聚（3HB-co-3HV）组成的共聚酯，作为唯一的其他成分。聚（SHB-co-3HV）是由果糖、葡萄糖酸盐、琥珀酸盐、醋酸盐或乳酸盐在细胞饥饿的氮、硫或镁源中产生的。虽然3HV仅占聚酯成分的8mol %，但本研究为聚（3HB）积累细菌突变体的构建和利用提供了基本原理，这些突变体改变了支链氨基酸的代谢，从而从单一不相关的底物中生产聚（3HB-co-3HV）。
Alcaligenes eutrophusmutant strain R3, which is a spontaneous revertant to prototrophy of an isoleucine-auxotrophic mutant of the wild-type strain H16, accumulated a copolyester consisting of 3-hydroxybutyric acid (3HB) as main constituent and of 3-hydroxyvaleric acid (3HV), i.e. poly(3HB-co-3HV), as the only other constituent from various single unrelated substrates, which were provided in excess, after a nutrient essential for growth was depleted in the medium. Poly(SHB-co-3HV) was produced from fructose, gluconate, succinate, acetate or lactate during cell starvation of the nitrogen, sulphur or magnesium source. Although 3HV amounted to only 8 mol% of the constituents of the polyester, this study provides a general rationale for construction and utilization of mutants of poly(3HB)-accumulating bacteria that are altered in the metabolism of branched-chain amino acids for the production of poly(3HB-co-3HV) from single unrelated substrates.