Electrical behaviour of dendritic spines as revealed by voltage imaging.
Electrical behaviour of dendritic spines as revealed by voltage imaging.
复制标题
电压成像揭示的树突状刺的电行为。
DOI：
10.1038/ncomms9436
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发表时间：
2015-10-05
影响因子：
16.6
通讯作者：
Zecevic D
中科院分区：
文献类型：
--
作者：
Popovic MA;Carnevale N;Rozsa B;Zecevic D
单个神经元上成千上万的树突棘通过复杂的传递受体和电压敏感离子通道分子组合产生电信号来处理信息和调节可塑性。然而，我们对脊髓电学行为的理解是有限的，因为不可能以足够的灵敏度和时空分辨率记录来自这些结构的输入信号。目前对间接数据的解释和基于理论考虑的推测是不确定的。在这里，我们使用一种电致变色电压敏感染料，它作为一个线性刻度的跨膜光学电压表，直接监测来自薄基树突上单个棘的电信号。结果表明，这些棘上的突触在很大程度上没有被棘颈电隔离。电学上，它们的行为就好像它们直接位于树突上。位于单个神经元上的树突棘处理信息，但我们对棘的电行为的理解仍然有限。在这里，作者使用电压敏感染料成像技术来监测来自细基底棘的电信号，并表明突触并不是被棘颈电隔离的。
Thousands of dendritic spines on individual neurons process information and mediate plasticity by generating electrical input signals using a sophisticated assembly of transmitter receptors and voltage-sensitive ion channel molecules. Our understanding, however, of the electrical behaviour of spines is limited because it has not been possible to record input signals from these structures with adequate sensitivity and spatiotemporal resolution. Current interpretation of indirect data and speculations based on theoretical considerations are inconclusive. Here we use an electrochromic voltage-sensitive dye which acts as a transmembrane optical voltmeter with a linear scale to directly monitor electrical signals from individual spines on thin basal dendrites. The results show that synapses on these spines are not electrically isolated by the spine neck to a significant extent. Electrically, they behave as if they are located directly on dendrites. Dendritic spines located on individual neurons process information, but our understanding of the electrical behaviour of spines is still limited. Here, the authors use voltage-sensitive dye imaging techniques to monitor electrical signals from thin basal spines and show that synapses are not electrically isolated by the spine neck.