Identification of type I microsatellite markers associated with genes and ESTs in Scylla paramamosain
Identification of type I microsatellite markers associated with genes and ESTs in Scylla paramamosain
复制标题
拟青青蟹基因和EST相关的I型微卫星标记的鉴定
DOI：
10.1016/j.bse.2011.05.007
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发表时间：
2011-08-01
影响因子：
1.6
通讯作者：
Ma, Lingbo
中科院分区：
文献类型：
--
作者：
Ma, Hongyu;Ma, Chunyan;Ma, Lingbo
本研究从Scylla paramamosain及其近缘种Scylla serrata的基因和ESTs中筛选微卫星。共检测到18个多态性位点，共106个等位基因。每个位点的观察杂合度和期望杂合度分别为0.08 ~ 1.00和0.08 ~ 0.88。多态性信息含量（PIC）显示12个位点具有高信息性（PIC >.5）。经Bonferroni校正后，发现有两个位点明显偏离Hardy-Weinberg平衡（P < 0.0028）。经Bonferroni校正后，Scpa-ALF-SSR位点与Scpa-SSR03位点之间存在显著连锁不平衡（P < 0.0003）。对这18个微卫星标记进行跨种扩增，结果表明，大多数标记在serrata s.s serrata、Scylla tranquebarica和Scylla olivacea中均能得到预期产物。本研究将为双歧杆菌及其相关类群遗传连锁图谱的构建、数量性状位点（QTL）的定位和比较基因组分析提供依据。(C) 2011 Elsevier Ltd.版权所有。
In this study, Microsatellites were screened from genes and ESTs of Scylla paramamosain and the related species Scylla serrata. Eighteen polymorphic loci were detected, with a total of 106 alleles. The observed and expected heterozygosity per locus ranged from 0.08 to 1.00, and from 0.08 to 0.88. Polymorphism information content (PIC) showed that 12 loci were high informative (PIC > 0.5). Two loci were found to deviate significantly from Hardy-Weinberg equilibrium after Bonferroni correction (P < 0.0028). Significant linkage disequilibrium was detected between loci Scpa-ALF-SSR and Scpa-SSR03 after Bonferroni correction (P < 0.0003). Cross-species amplification of these 18 microsatellite markers showed that most of them could give the expected products in three other species: S. serrata, Scylla tranquebarica and Scylla olivacea. This study will be useful for construction of genetic linkage maps, mapping of quantitative trait loci (QTL), and analysis of comparative genome in S. paramamosain and the related taxa. (C) 2011 Elsevier Ltd. All rights reserved.