Detection of endocrine disrupters: Evaluation of a Fish Sexual Development Test (FSDT)
Detection of endocrine disrupters: Evaluation of a Fish Sexual Development Test (FSDT)
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DOI：
10.1016/j.cbpc.2006.05.006
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发表时间：
2006-09-01
影响因子：
3.9
通讯作者：
Bjerregaard, Poul
中科院分区：
文献类型：
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作者：
Holbech, Henrik;Kinnberg, Karin;Bjerregaard, Poul
在经济合作与发展组织（经合组织）的管理下，许多实验室开展了一项全面的工作，以制定检测人类和各种动物物种内分泌干扰化学品的测试准则。这项工作的一部分是开发检测鱼类中具有内分泌干扰特性的化学品的测试方法。鱼类性发育测试（FSDT）（现有OECD TG 210，鱼类早期生命阶段毒性测试的扩展），被提议作为检测内分泌干扰化学物质的国际测试指南，通过幼年斑马鱼在水中暴露于三种天然雌激素进行评估：雌酮、17 β-雌二醇、雌三醇和合成雄激素群勃龙（醋酸群勃龙）。作为终点，研究了卵黄蛋白原诱导和组织学变化，包括性别比的变化。雌酮、17 β-雌二醇和雌三醇分别为49纳克/升、54纳克/升和22微克/升时，雌性的性别比发生了显著变化。从暴露于9.7 ng/l及以上的群勃龙中观察到所有男性人群。在暴露于14 ng/l雌酮、54 ng/l 17 β-雌二醇和0.6 μ g/l雌三醇后，分别测量到全身匀浆中显著的卵黄蛋白原诱导。暴露于193 ng/l或更高浓度的群勃龙后，测量到卵黄蛋白原显著减少。目前的结果提供了强有力的证据，FSDT是一个敏感的测试对雌激素，特别是雄激素的暴露和验证的FSDT作为经合组织的测试指南应继续。(c)2006年爱思唯尔公司All rights reserved.
Managed by the Organisation for Economic Co-operation and Development (OECD), a comprehensive work is carried out in numerous laboratories to develop test guidelines for the detection of endocrine disrupting chemicals in humans, and various animal species. Development of tests to detect chemicals with endocrine disrupting properties in fish is a part of that work. A Fish Sexual Development Test (FSDT) (an extension of the existing OECD TG 210, fish early life stage toxicity test), proposed as an international test guideline for the detection of endocrine disrupting chemicals, was evaluated by water exposure of juvenile zebrafish to the three natural estrogens: estrone, 17 beta-estradiol, and estriol and the synthetic androgen trenbolone (trenbolone acetate). As endpoints, vitellogenin induction and histological changes including changes in sex ratios were investigated. The sex ratio was significantly altered towards females from 49 ng/l estrone, 54 ng/l 17 beta-estradiol and 22 mu g/l estriol, respectively. An all male population was observed from exposure to 9.7 ng/l trenbolone and above. Significant vitellogenin induction in whole body homogenate was measured after exposure to 14 ng/l estrone, 54 ng/l 17 beta-estradiol and 0.6 mu g/l estriol, respectively. Significant vitellogenin reduction was measured after exposure to 193 ng/l trenbolone or higher. The present results provide strong evidence that the FSDT is a sensitive test toward estrogenic and especially androgenic exposure and the validation of the FSDT as an OECD test guideline should continue. (c) 2006 Elsevier Inc. All rights reserved.