A new technique to expand human mesenchymal stem cells using basement membrane extracellular matrix
A new technique to expand human mesenchymal stem cells using basement membrane extracellular matrix
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DOI：
10.1016/j.bbrc.2003.11.143
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发表时间：
2004-01-16
影响因子：
3.1
通讯作者：
Kato, Y
中科院分区：
文献类型：
--
作者：
Matsubara, T;Tsutsumi, S;Kato, Y
间充质干细胞（MSC）显示出非常短的增殖寿命，并且在培养中容易失去分化潜能。然而，使用由PYS-2细胞或原代内皮细胞产生的基底膜样细胞外基质包被的组织培养皿，干细胞的生长速率和增殖寿命显著增加。此外，干细胞在细胞外基质上扩增，而不是在塑料组织培养皿上扩增，在许多有丝分裂中保留了成骨、软骨形成和脂肪形成的潜力。细胞外基质比碱性成纤维细胞生长因子更能促进MSC的增殖和维持其多向分化潜能。在细胞外基质上扩增的间充质干细胞应该可用于大组织缺损的再生和重复的细胞治疗，这需要大量的干细胞或祖细胞。(C)2003年爱思唯尔公司All rights reserved.
Mesenchymal stem cells (MSC) show a very short proliferative life span and readily lose the differentiation potential in culture. However, the growth rate and the proliferative life span of the stem cells markedly increased using tissue culture dishes coated with a basement membrane-like extracellular matrix, which was produced by PYS-2 cells or primary endothelial cells. Furthermore, the stem cells expanded on the extracellular matrix, but not those on plastic tissue culture dishes, retained the osteogenic, chondrogenic, and adipogenic potential throughout many mitotic divisions. The extracellular matrix had greater effects on the proliferation of MSC and the maintenance of the multi-lineage differentiation potential than basic fibroblast growth factor. Mesenchymal stem cells expanded on the extracellular matrix should be useful for regeneration of large tissue defects and repeated cell therapies, which require a large number of stem or progenitor cells. (C) 2003 Elsevier Inc. All rights reserved.