Abundance of Candidatus 'Accumulibacter phosphatis' in enhanced biological phosphorus removal activated sludge acclimatized with different carbon sources
Abundance of Candidatus 'Accumulibacter phosphatis' in enhanced biological phosphorus removal activated sludge acclimatized with different carbon sources
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DOI：
10.1264/jsme2.22.346
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发表时间：
2007-12-01
影响因子：
2.2
通讯作者：
Mino, Takashi
中科院分区：
文献类型：
--
作者：
Fukushima, Toshikazu;Uda, Naoki;Mino, Takashi
在本研究中，在5个强化生物除磷（EBPR）活性污泥反应器中，与不同的碳源运行的丰度和聚磷酸盐的积累的Accumulibacter phosphatis的调查。荧光原位杂交（FISH）结合4 '，6-二脒基-2-苯基吲哚（DAPI）染色的聚磷酸盐颗粒证实了聚磷酸盐的积累，在所有的反应器中的腐殖酸'积累磷杆菌'。从FISH图像中确定了磷酸盐累积杆菌的丰度。当EBPR活性高，磷含量占混合液挥发性悬浮固体（MLVSS）的9%或以上时，在用乙酸盐、天冬氨酸盐或葡萄糖驯化的反应器中，磷酸盐积累杆菌占真细菌的20%以上。而在主要用酵母提取物、蛋白胨驯化的反应器中，该值低至约10%。或谷氨酸盐。在这些反应器中，附属于放线菌的细菌被发现积累聚磷酸盐，并有助于除磷。磷酸积累杆菌（Accumulibacter phosphatis）利用多种碳源参与除磷，但其丰度因碳源类型而异。
In the present study, the abundance of Candidatus 'Accumulibacter phosphatis' and the accumulation of polyphosphate were investigated in five enhanced biological phosphorus removal (EBPR) activated sludge reactors operated with different carbon sources. Fluorescence in situ hybridization (FISH) in combination with 4',6-diamidino-2-phenylindole (DAPI) staining for polyphosphate granules confirmed the accumulation of polyphosphate by Candidatus 'Accumulibacter phosphatis' in all the reactors. The abundance of Candidatus 'Accumulibacter phosphatis' was determined from the FISH images. When EBPR activity was high and phosphorus content made up around 9% or more of mixed liquor volatile suspended solids (MLVSS), Candidatus 'Accumulibacter phosphatis' accounted for over 20% of the eubacteria in the reactors acclimatized with acetate, aspartate, or glucose. Whereas this value was as low as around 10% in the reactors acclimatized mainly with yeast extract, peptone.. or glutamate. In these reactors, bacteria affiliated with Actinobacteria were found to accumulate polyphosphate and to contribute to phosphorus removal. Candidatus 'Accumulibacter phosphatis' takes part in the removal of phosphorus by using various carbon sources, but its abundance varies according to the type of carbon source.