13C-NMR studies of selectively carboxymethylated [methyl-13C]methionine-labeled bacterial dihydrofolate reductase.
13C-NMR studies of selectively carboxymethylated [methyl-13C]methionine-labeled bacterial dihydrofolate reductase.
复制标题
选择性羧甲基化[甲基-13C]蛋氨酸标记的细菌二氢叶酸还原酶的 13C-NMR 研究。
DOI：
10.1016/0014-5793(83)80935-1
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发表时间：
1983
期刊：
影响因子：
3.5
通讯作者：
Blakley,RL
中科院分区：
文献类型：
--
作者：
London,RE;Wageman,WE;Blakley,RL
在用于NMR研究的相对温和的条件下，通过使未标记的碘乙酸盐与含有生物合成掺入的（甲基-/sup 13/C）甲硫氨酸的酶反应，制备/sup 13/C标记的屎链球菌酶。通过这种独特的策略产生的化合物被用作二氢叶酸还原酶的持续研究的一部分。NMR研究提供了两个甲硫氨酸残基的共振数据归属。此外，羧甲基化的特异性为共振归属提供了有用的基础。
The /sup 13/C-labeled Streptococcus faecium enzyme was prepared under relatively mild conditions for NMR studies by reaction of unlabeled iodoacetate with the enzyme containing biosynthetically incorporated (methyl-/sup 13/C)methionine. The compound produced by this unique strategy was used as a part of a continuing study of the enzyme dihydrofolate reductase. The NMR studies provided assignment of resonance data for two of the methionine residues. Additionally, the specificity of the carboxymethylation provides a useful basis for resonance assignment.