A Th1/Th2-associated chemokine imbalance during infancy in children developing eczema, wheeze and sensitization
A Th1/Th2-associated chemokine imbalance during infancy in children developing eczema, wheeze and sensitization
复制标题
DOI：
10.1111/j.1365-2222.2011.03827.x
复制
发表时间：
2011-12-01
影响因子：
6.1
通讯作者：
Jenmalm, M. C.
中科院分区：
文献类型：
--
作者：
Abrahamsson, T. R.;Abelius, M. Sandberg;Jenmalm, M. C.
背景 循环趋化因子的分析为研究体内过敏性疾病中 T 辅助细胞 (Th)1/Th2 失衡提供了新的工具。目的 将婴儿期循环 Th1 和 Th2 相关趋化因子与过敏性疾病、致敏和益生菌补充剂联系起来。方法 Th1 相关 CXC 趋化因子配体 (CXCL) 9、CXCL10 和 CXCL11 以及 Th2 相关的循环水平在 161 名婴儿出生时 (n = 109)、6 (n = 104)、12 (n = 116) 和 24 个月 (n = 123) 中，使用 Luminex 和 CCL18 进行酶联免疫吸附测定，评估 CC 趋化因子配体 (CCL)17 和 CCL22，这些婴儿在 2017 年最后一个月完成了罗伊氏乳杆菌双盲安慰剂对照过敏预防试验。妊娠期和生命的第一年。随访婴儿2岁时过敏性疾病和致敏的发生情况。结果Th2相关趋化因子CCL17和CCL22在出生时最高，然后下降，而CCL18和Th1相关趋化因子随着年龄的增长而增加。在患有过敏性疾病的儿童中观察到高 Th2 相关趋化因子水平。致敏之前，出生时 Th2 相关的 CCL22 水平就会升高，而 Th1 相关的 CXCL11 水平就会降低。患有反复喘息的婴儿出生时，Th2 相关的 CCL17 也升高。出生时较高的 Th2/Th1 比率 (CCL22/CXCL10) 与致敏和湿疹的发生有关。出生第一周粪便中存在罗伊氏乳杆菌与 6 个月大时 CCL17 和 CCL22 水平较低以及 CXCL11 水平较高相关。高 Th1 相关趋化因子水平与日托相关。结论和临床相关性 婴儿期过敏性疾病和过敏与出生后循环 Th1 相关趋化因子水平低和高 Th2 相关趋化因子水平相关。循环趋化因子可用于研究体内过敏性疾病中 Th1/Th2 失衡。阐明趋化因子在过敏性疾病中的作用可能会导致未来的治疗（ClinicalTrials.gov NCT01285830）。
Background Analyses of circulating chemokines offer novel tools to investigate the T helper (Th)1/Th2 imbalance in allergic disease in vivo.Objective To relate circulating Th1- and Th2-associated chemokines in infancy to allergic disease, sensitization and probiotic supplementation.Methods Circulating levels of Th1-associated CXC-chemokine ligand (CXCL) 9, CXCL10 and CXCL11 and Th2-associated CC-chemokine ligand (CCL)17 and CCL22 were assessed with Luminex and CCL18 with enzyme-linked immunosorbent assay at birth (n = 109), 6 (n = 104), 12 (n = 116) and 24 months (n = 123) in 161 infants completing a double-blind placebo-controlled allergy prevention trial with Lactobacillus reuteri during the last month of gestation and through the first year of life. The infants were followed regarding the development of allergic disease and sensitization until 2 years of age.Results The Th2-associated chemokines CCL17 and CCL22 were the highest at birth and then decreased, whereas CCL18 and the Th1-associated chemokines increased with age. High Th2-associated chemokine levels were observed in children developing allergic disease. Sensitization was preceded by elevated levels of the Th2-associated CCL22 and reduced levels of the Th1-associated CXCL11 already at birth. The Th2-associated CCL17 was also elevated at birth in infants developing recurrent wheeze. A high Th2/Th1 ratio (CCL22/CXCL10) at birth associated with both sensitization and eczema development. The presence of L. reuteri in stool in the first week of life was associated with low CCL17 and CCL22 and high CXCL11 levels at 6 months of age. High Th1-associated chemokine levels were associated with day-care.Conclusion and Clinical Relevance Allergic disease and sensitization in infancy was associated with low circulating Th1- and high Th2-associated chemokine levels already from birth. Circulating chemokines are useful for investigating the Th1/Th2 imbalance in allergic disease in vivo. Elucidation of the role of chemokines in allergic diseases may lead to future treatments (ClinicalTrials. gov NCT01285830).