Characterization of the subcellular localization of herpes simplex virus type 1 proteins in living cells
Characterization of the subcellular localization of herpes simplex virus type 1 proteins in living cells
复制标题
活细胞中单纯疱疹病毒 1 型蛋白亚细胞定位的表征
DOI：
10.1007/s00430-010-0175-9
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发表时间：
2011-02-01
影响因子：
5.4
通讯作者：
Zheng, Chunfu
中科院分区：
文献类型：
--
作者：
Xing, Junji;Wang, Shuai;Zheng, Chunfu
在这项研究中，我们构建了单纯疱疹病毒1型(HSV-1)蛋白质组的表达克隆文库，并利用黄色荧光蛋白(YFP)融合蛋白绘制了HSV-1蛋白在活细胞中的亚细胞定位图。结果表明，21个蛋白质呈胞浆或亚胞质定位，16个蛋白质呈核或亚核定位，其余蛋白质同时存在于胞核和胞浆中。有趣的是，大多数衣壳蛋白在细胞核中表现出丰富或排他性的定位，而大多数包膜蛋白则表现为细胞质定位，这表明这些蛋白的亚细胞定位与它们在病毒复制过程中的功能有关。这些结果提供了HSV-1蛋白在活细胞中的亚细胞定位图，为进一步研究这些蛋白的功能提供了有用的信息。
In this study, we presented the construction of a library of expression clones for the herpes simplex virus type 1 (HSV-1) proteome and subcellular localization map of HSV-1 proteins in living cells using yellow fluorescent protein (YFP) fusion proteins. As a result, 21 proteins showed cytoplasmic or subcytoplasmic localization, 16 proteins showed nuclear or subnuclear localization, and others were present both in the nucleus and cytoplasm. Interestingly, most capsid proteins showed enriched or exclusive localization in the nucleus, and most of the envelope proteins showed cytoplasmic localization, suggesting that subcellular localization of the proteins correlated with their functions during virus replication. These results present a subcellular localization map of HSV-1 proteins in living cells, which provide useful information to further characterize the functions of these proteins.