Retinal glial cell immunoreactivity and neuronal cell changes in rats with STZ-induced diabetes
Retinal glial cell immunoreactivity and neuronal cell changes in rats with STZ-induced diabetes
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DOI：
10.1076/ceyr.23.4.276.5459
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发表时间：
2001-01-01
影响因子：
2
通讯作者：
Agardh, CD
中科院分区：
文献类型：
--
作者：
Agardh, E;Bruun, A;Agardh, CD
目的。研究糖尿病是否会影响神经胶质细胞、视网膜神经元和色素上皮细胞，如果是的话，评估氨基胍 (AG) 或普罗布考 (PB) 是否会影响任何变化。方法。链脲佐菌素 (STZ) 诱导的糖尿病雄性 Wistar 大鼠和年龄匹配的对照大鼠喂养正常饮食，在饮用水中添加 AG（糖尿病大鼠为 0.5 g/l，对照大鼠为 1.0 g/l）或颗粒中添加 PB（1% w/w），持续 1 或 6 个月。石蜡包埋的视网膜切片分别在胶质细胞、水平细胞、色素上皮细胞和神经节细胞的一抗 GFAP、钙结合蛋白、RPE65 和 Hu 中孵育，并在荧光二抗中孵育。结果。注射 STZ 1 个月后，GFAP 免疫反应性稀疏，但 6 个月后，5/5 糖尿病患者和 1/7 对照视网膜的神经胶质细胞中 GFAP 免疫反应性显着（p=0.015）。 AG 和 PB 都不影响这种免疫反应性。糖尿病 1 个月和 6 个月时，视网膜色素上皮细胞和神经节细胞层细胞的数量相似。随着时间的推移，水平单元的数量减少（p
Purpose. To study whether diabetes could influence glial cells, retinal neurons, and pigment epithelial cells and if so, to evaluate whether any changes could be influenced by aminoguanidine (AG) or probucol (PB).Methods. Streptozocin (STZ)-induced diabetic male Wistar rats and age-matched control rats were fed a normal diet, addition of AG in the drinking water (0.5 g/l for diabetic and 1.0 g/l for control rats) or PB in the pellets (1% w/w) for one or six months. Paraffin embedded retinal sections were incubated in the primary antibodies GFAP, calbindin, RPE65, and Hu, for glial, horizontal, pigment epithelial, and ganglion cells, respectively, and in fluorescent secondary antibodies.Results. One month after STZ injection, GFAP immunoreactivity was sparse, but after six months it was prominent in glial cells in 5/5 diabetic and 1/7 control retinas (p=0.015). Neither AG, nor PB influenced this immunoreactivity. Numbers of retinal pigment epithelial cells and cells in the ganglion cell layer, were similar at one and six months of diabetes. By time, the number of horizontal cells decreased (p