In vivo repair of cytosine hydrates in DNA of cultured human lymphoblasts.
In vivo repair of cytosine hydrates in DNA of cultured human lymphoblasts.
复制标题
培养的人淋巴母细胞 DNA 中胞嘧啶水合物的体内修复。
DOI：
10.1111/j.1751-1097.1993.tb09552.x
复制
发表时间：
1993
影响因子：
3.3
通讯作者：
Gallagher,PE
中科院分区：
文献类型：
--
作者：
Weiss,RB;Gallagher,PE
Ultraviolet irradiation of DNAin vitroresults in the production of a wide variety of pyrimidine base alterations, including cytosine hydrates. Enzymes that initiate the repair of monomeric pyrimidine damage have been identified in both bacterial and mammalian systems; however, thein vivoformation and repair of cytosine photohydrates has not been demonstrated in cellular DNA. UsingEscherichia coliendonuclease III as a damage‐specific probe, we have shown that ring‐saturated pyrimidines are formed in cultured human cells by irradiation with broadspectrum UV light. In addition, these types of base damage are removed from the DNA of human lymphoblasts within 5 h following the irradiation. Analysis of the action spectrum for the formation of cytosine hydrates in DNA reveals that these photoproducts are formed most efficiently by irradiation in the range of 255–265 nm light, coinciding with the wavelengths that are maximally absorbed by the DNA bases.
