CXCR4-targeted near-infrared imaging allows detection of orthotopic and metastatic human osteosarcoma in a mouse model.
CXCR4-targeted near-infrared imaging allows detection of orthotopic and metastatic human osteosarcoma in a mouse model.
复制标题
CXCR4 靶向近红外成像可以在小鼠模型中检测原位和转移性人类骨肉瘤。
DOI：
10.1038/srep15244
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发表时间：
2015-10-16
影响因子：
4.6
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Guan G;Lu Y;Zhu X;Liu L;Chen J;Ma Q;Zhang Y;Wen Y;Yang L;Liu T;Wang W;Ran H;Qiu X;Ke S;Zhou Y
CXCR 4在骨肉瘤的原发和转移部位表达。我们开发了一种新型的CXCR 4靶向近红外（NIR）荧光成像剂（简称CXCR 4-IR-783）。检查了与代表性骨肉瘤细胞（高和低CXCR 4表达的F5 M2和F4）的结合。还使用NIR荧光显微镜和Kodakin-vivomultispectrum系统在人骨肉瘤的小鼠异种移植模型中检查CXCR 4-IR-783荧光。采用显微CT、18F-PET扫描和近红外成像扫描检测荷瘤小鼠肺转移瘤。简言之，CXCR 4-IR-783的结合在F5 M2中显著高于在F4细胞中。在骨肉瘤异种移植物中检测到强烈的NIR荧光信号，在携带F5 M2细胞的小鼠中信号/背景比为4.87。接种F5 M2细胞4周后，CXCR 4-IR-783和micro-CT扫描可检测到肺转移灶，但18F-FDG PET扫描不能检测到。总之，CXCR 4-IR-783是检测高CXCR 4表达的骨肉瘤，特别是其转移性病变的一种有前途的工具。
CXCR4 is expressed at primary and metastatic sites of osteosarcoma. We developed a novel CXCR4-targeted near-infrared (NIR) fluorescent imaging agent (referred to as CXCR4-IR-783). The binding to representative osteosarcoma cells (F5M2 and F4 for high- and low- CXCR4 expression) was examined. CXCR4-IR-783 fluorescence was also examined in a mouse xenograft model of human osteosarcoma using NIR fluorescence microscopy and a Kodakin-vivomultispectral system. Pulmonary metastases in mice bearing osteosarcoma xenografts were detected by micro CT,18F-PET scan and NIR imaging scan. Briefly, the binding of CXCR4-IR-783 was significantly higher in F5M2 than in F4 cells. Intense NIR fluorescence signals were detected in osteosarcoma xenografts, with signal/background ratio at 4.87 in mice bearing the F5M2 cell. At 4 weeks after F5M2 cell inoculation, metastatic lesions in the lungs were detectable using CXCR4-IR-783 and micro-CT scan, but not with18F-FDG PET scan. In conclusion, CXCR4-IR-783 is a promising tool for detection of high CXCR4-expressing osteosarcoma and particularly for its metastatic lesions.