Understanding solid-phase HLA antibody assays and the value of MFI.
Understanding solid-phase HLA antibody assays and the value of MFI.
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DOI：
10.1016/j.humimm.2017.05.007
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发表时间：
2017-07
期刊：
影响因子：
2.7
通讯作者：
Bray RA
中科院分区：
文献类型：
--
作者：
Sullivan HC;Gebel HM;Bray RA
随着医学实践越来越依赖影像学和实验室测试，医疗决策将越来越依赖于数字。因此，在 HLA 检测体系中引入固相检测后，实验室主任和医生采用预设的平均荧光强度 (MFI) 阈值作为移植患者管理决策的基础。然而，MFI 值意味着什么？文献中充斥着详细介绍影响抗体评估的众多因素的报告，包括（但不限于）患者的致敏史、供体和受体之间的不匹配程度、血清中干扰物质的存在、多重微珠上的抗原是天然的还是变性的、日常和技术人员的变化以及给定机构中检测的历史表现。这些变量如何纳入 MFI 值的解释中？在此，讨论了单抗原珠 (SAB) 测试和解释的陷阱和复杂性，特别关注 MFI 可以推断什么和不能推断什么。
As the practice of medicine becomes more reliant on imaging and laboratory tests, medical decisions will be increasingly based on numbers. Accordingly, following the introduction of solid-phase testing to the HLA testing repertoire, laboratory directors and physicians have employed preset mean fluorescence intensity (MFI) thresholds as the basis for decisions in the management of transplant patients. However, what do MFI values mean? The literature is rife with reports detailing numerous factors that influence antibody assessment including (but not limited to) sensitization history of the patient, level of mismatch between donor and recipient, presence of interfering substances in the serum, whether the antigen on multiplex beads is native or denatured, day-to-day and technologist variability, and the historical performance of an assay in a given institution. How are these variables incorporated into the interpretation of MFI values? Herein, the pitfalls and complexities of single antigen bead (SAB) testing and interpretation are discussed with specific attention to what can and cannot be inferred by MFI.
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影响因子：
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作者：
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