Multi-color imaging of fluorescent nanodiamonds in living HeLa cells using direct electron-beam excitation.
Multi-color imaging of fluorescent nanodiamonds in living HeLa cells using direct electron-beam excitation.
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DOI：
10.1002/cphc.201300802
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发表时间：
2014-03
期刊：
影响因子：
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通讯作者：
Y. Nawa;W. Inami;Sheng Lin;Y. Kawata;S. Terakawa;C. Fang;Huan‐Cheng Chang
中科院分区：
文献类型：
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作者：
Y. Nawa;W. Inami;Sheng Lin;Y. Kawata;S. Terakawa;C. Fang;Huan‐Cheng Chang
利用直接电子束激发辅助荧光(D-EXA)显微镜对活体HeLa细胞中的荧光纳米钻石(FND)进行了多色、高空间分辨率成像。在这种技术中，荧光材料被聚焦的电子束直接激发，所产生的阴极发光(CL)以纳米级的分辨率被检测到。利用发射绿光和红光的FND进行双色成像，在具有高空间分辨率的细胞中同时观察到它们。该技术可广泛应用于多种细胞功能的多色免疫染色。
Multi-color, high spatial resolution imaging of fluorescent nanodiamonds (FNDs) in living HeLa cells has been performed with a direct electron-beam excitation-assisted fluorescence (D-EXA) microscope. In this technique, fluorescent materials are directly excited with a focused electron beam and the resulting cathodoluminescence (CL) is detected with nanoscale resolution. Green- and red-light-emitting FNDs were employed for two-color imaging, which were observed simultaneously in the cells with high spatial resolution. This technique could be applied generally for multi-color immunostaining to reveal various cell functions.