Myoinformatics report: myosin regulatory light chain paralogs in the human genome
Myoinformatics report: myosin regulatory light chain paralogs in the human genome
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DOI：
10.1007/s10974-006-9057-6
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发表时间：
2006-02-01
影响因子：
2.7
通讯作者：
Collins, John H.
中科院分区：
文献类型：
--
作者：
Collins, John H.
在线生物信息学工具被用来识别和表征所有的旁系同源物（基因组内同源物）的肌肉肌球蛋白调节轻链在人类基因组中。最初的搜索产生了22个可能的旁系同源物，但仔细检查支持数据排除了其中的大部分。五个旁系同源物被清楚地鉴定出其表达的组织类型（骨骼肌和心肌、平滑肌、非肌肉细胞质）。序列比较和系统发育分析表明，平滑肌和非肌肉旁系同源物的共同祖先的骨骼肌和心肌旁系同源物的共同祖先的早期分歧。一个不寻常的第六人类paradenosine是非常相似的“超快”肌球蛋白的监管轻链，迄今为止已被发现只在猫。最后，一个独特的和可疑的“前体淋巴细胞”paradenosine初步确定。以鸡快速骨骼肌调节轻链的已知结构为模板，构建了所有7个人类旁系同源物的三维结构模型。
On-line bioinformatics tools were used to identify and characterize all paralogs (intragenomic homologs) of muscle myosin regulatory light chains in the human genome. The initial search yielded 22 possible paralogs, but careful examination of supporting data eliminated most of these. Five paralogs were clearly identified with the tissue types (skeletal and cardiac muscles, smooth muscle, non-muscle cytoplasm) in which they are expressed. Sequence comparisons and phylogenetic analysis showed early divergence of a common ancestor of smooth muscle and non-muscle paralogs from a common ancestor of skeletal and cardiac muscle paralogs. An unusual sixth human paralog was very similar to the regulatory light chain of "superfast" myosin, which to date has been found only in cat. Finally, a unique and questionable "precursor lymphocyte" paralog was tentatively identified. Three-dimensional structural models of all seven human paralogs were constructed using the known structure of chicken fast skeletal muscle regulatory light chain as a template.