DEAH box RNA helicase DHX38 associates with satellite I noncoding RNA involved in chromosome segregation
DEAH box RNA helicase DHX38 associates with satellite I noncoding RNA involved in chromosome segregation
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DOI：
10.1111/gtc.12707
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发表时间：
2019-06-28
期刊：
影响因子：
2.1
通讯作者：
Ideue, Takashi
中科院分区：
文献类型：
--
作者：
Nishimura, Kanako;Cho, Yukiko;Ideue, Takashi
称为卫星I的非编码RNA是从人类着丝粒区域转录而来的。由于染色体分离缺陷，这种ncRNA的缺失导致核形态异常。一些蛋白质因子与ncRNA相互作用，并在有丝分裂调节中作为nc核糖核蛋白（RNP）复合物的组成部分发挥作用。在这里，我们发现DHX38，一个pre-mRNA剪接相关的DEAH盒RNA解旋酶，与卫星I ncRNA相互作用。DHX38缺失导致染色体分离缺陷，类似于敲低卫星I ncRNA。DHX38与ncRNA的相互作用是期间特异性的，但DHX38的缺失影响了有丝分裂期与卫星I ncRNA相关的Aurora B的功能。基于这些发现，我们认为DHX38作为间期卫星I ncRNP复合体的一个组成部分在有丝分裂调控中起作用。
Noncoding (nc) RNA called satellite I is transcribed from the human centromere region. Depletion of this ncRNA results in abnormal nuclear morphology because of defects in chromosome segregation. Some protein factors interact with this ncRNA and function as a component of a nc ribonucleoprotein (RNP) complex in mitotic regulation. Here, we found that DHX38, a pre-mRNA splicing-related DEAH box RNA helicase, interacts with satellite I ncRNA. Depletion of DHX38 resulted in defective chromosome segregation similar to knockdown of satellite I ncRNA. Interaction between DHX38 and ncRNA was interphase-specific, but DHX38 depletion affected the function of Aurora B, which associated with satellite I ncRNA at mitotic phase. Based on these findings, we suggest that DHX38 has a role in mitotic regulation as a component of the satellite I ncRNP complex at interphase.