Evaluation of a new reagent: Anthraquinone-2-sulfonyl chloride for the determination of phenol in water by liquid chromatography using precolumn phase-transfer catalyzed derivatization
Evaluation of a new reagent: Anthraquinone-2-sulfonyl chloride for the determination of phenol in water by liquid chromatography using precolumn phase-transfer catalyzed derivatization
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DOI：
10.1093/chromsci/41.7.337
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发表时间：
2003-08-01
影响因子：
1.3
通讯作者：
An, DK
中科院分区：
文献类型：
--
作者：
Feng, F;Uno, B;An, DK
报道了一种新试剂--2-苯醌-2-磺酰氯用于苯酚的衍生化反应。选择了几种不同极性的化合物来评价通过简单途径制备的新试剂和这些酚的衍生物。讨论了分析衍生化的最佳条件和衍生化反应的机理。衍生化过程包括用有机相中的四丁基铵反离子对去质子化的苯酚进行离子对萃取。在两相界面上，在室温下，衍生化反应在3min内定量发生。这些衍生物是稳定的，易于用正相(NP)和反相(RP)高效液相色谱(HPLC)分析。在320 nm和256 nm处，NP-HPLC线性关系良好，线性范围为0.20~200umol/L。结合Waters Sep-Pak Plus C18色谱柱的预富集，水样分析中酚的检出限低至1×10−9mol/L(∼0.1微克/毫升)。
A new reagent, anthraquinone-2-sulfonyl chloride, is used for the derivatizaton of phenols. Several compounds with different polarities are selected to evaluate the new reagent and derivatives of these phenols that are prepared via a facile pathway. The optimal conditions for analytical derivatization and mechanism of the derivatization reaction are discussed. The derivatization procedure involves an ion-pair extraction of the deprotonated phenols with a tetrabutylammonium counter ion in the organic phase. At the interface of two phases, the derivatization reaction occurs quantitatively at room temperature within 3 min. The derivatives are stable and readily amenable to analysis by normal-phase (NP) and reversed-phase (RP) high-performance liquid chromatography (HPLC). Excellent linearity response was demonstrated over the concentration range of 0.2–200 µmol/L at 320 nm for NP-HPLC and at 256 nm for RP-HPLC. Combined with preconcentration using a Waters Sep-Pak Plus C18cartridge, detection limits of phenols for water-sample analysis are as low as 1 × 10−9mol/L (∼ 0.1 µg/mL).