Tumor necrosis factor-alpha and tumor necrosis factor receptors in the failing human heart
Tumor necrosis factor-alpha and tumor necrosis factor receptors in the failing human heart
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DOI：
10.1161/01.cir.93.4.704
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发表时间：
1996-02-15
期刊：
影响因子：
37.8
通讯作者：
Mann, DL
中科院分区：
文献类型：
--
作者：
TorreAmione, G;Kapadia, S;Mann, DL
背景 肿瘤坏死因子-α (TNF-α) 是一种促炎细胞因子，可在心脏中产生负性肌力作用。最近，据报道，晚期充血性心力衰竭患者中 TNF-α 水平升高。尽管 TNF-α 被认为通过与两种细胞表面受体 TNFR1 和 TNFR2 结合来发挥其有害作用，但心脏病状态下心脏中 TNF 受体的表达和调节水平尚不清楚。方法和结果器官捐献者以及终末期扩张型心肌病 (DCM) 和缺血性心脏病 (IHD) 患者的移植心脏中的 TNFR1、TNFR2 和 TNF-α 的 Northern 印迹分析显示，通过 ELISA 检测，在非衰竭心脏、DCM 和 IHD 心脏中，TNFR1 和 TNFR2 受体蛋白水平降低了 60%。拥有永不衰竭的心（P
Background Tumor necrosis factor-alpha (TNF-alpha is a proinflammatory cytokine that produces negative inotropic effects in the heart. Recently, elevated levels of TNF-alpha have been reported in patients with advanced congestive heart failure. Although TNF-alpha is thought to exert its deleterious effects by binding to two cell surface receptors, TNFR1 and TNFR2, the level of expression and regulation of TNF receptors in the heart in cardiac disease states is not known.Methods and Results We examined mRNA and protein levels for TNFR1, TNFR2, and TNF-alpha in explanted hearts from organ donors as well as in patients with end-stage dilated cardiomyopathy (DCM) and ischemic heart disease (IHD). Northern blot analysis revealed that mRNA for TNFR1 and TNFR2 was present in nonfailing, DCM, and IHD hearts. TNFR1 and TNFR2 receptor protein levels, as measured by ELISA, were decreased 60% in DCM and IHD patients compared with nonfailing hearts (P