Effect of cooling on the responsiveness of canine tracheal muscle.
Effect of cooling on the responsiveness of canine tracheal muscle.
复制标题
冷却对犬气管肌肉反应性的影响。
DOI：
10.1164/ajrccm/142.6_pt_1.1402
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Irvin,CG
中科院分区：
文献类型：
--
作者：
Bethel,RA;Tanaka,DT;Mitchell,LS;Curtis,SP;Leff,AR;Irvin,CG
在许多哮喘患者中，气道冷却会导致支气管收缩。为了确定冷却对气管肌肉对副交感神经和毒蕈碱刺激反应的直接影响，在犬颈气管的一段上皮表面上使用温控盐水，将其温度调节到37、30或25℃。然后测定了气管肌肉对迷走神经电刺激和动脉内乙酰胆碱的收缩反应。将节段冷却至25℃可减少副交感神经刺激引起的收缩。然而，冷却没有改变动脉内乙酰胆碱引起的收缩。平行研究在体外进行，使用从另外三只狗的气管同一部分切除的9条气管肌肉条。体外将气管肌肉冷却至25℃时，电场刺激和乙酰胆碱诱导的最大收缩量均增加。因此，原位冷却不会改变气管肌肉对毒蕈碱刺激的反应，并抑制对副交感神经刺激的反应。这些数据表明，体外冷却引起的气管肌肉反应的增强并不能反映原位反应。他们认为，冷却对气道平滑肌对副交感神经和毒蕈碱刺激反应的直接影响并不能解释体内冷却诱导的支气管收缩。我于1990年重新呼吸；142: 1402 - 1406
Airway cooling causes bronchoconstriction in many persons who have asthma. To determine the direct effect of cooling on the response of tracheal muscle to parasympathetic and muscarinic stimuli in situ, the temperature of a segment of canine cervical trachea was adjusted to 37, 30, or 25 C by superfusing temperature-controlled saline over its epithelial surface. The contractile response of the tracheal muscle to electrical stimulation of the vagus nerves and to intra-arterial acetylcholine was then determined. Cooling the segment to 25 C decreased the contraction induced by parasympathetic stimulation. However, cooling did not alter the contraction induced by intra-arterial acetylcholine. Parallel studies were conducted in vitro using nine excised tracheal muscle strips taken from the same section of trachea in three additional dogs. Cooling the tracheal muscle to 25 C in vitro increased the maximal contraction induced by both electrical field stimulation and by acetylcholine. Thus, cooling in situ does not alter the response of tracheal muscle to muscarinic stimuli and inhibits the response to parasympathetic stimuli. These data indicate that augmentation of the tracheal muscle response elicited by cooling in vitro does not reflect the response in situ. They suggest that a direct effect of cooling on airway smooth muscle response to parasympathetic and muscarinic stimuli does not account for cooling-induced bronchoconstriction in vivo. AM REV RESPIR DIS 1990; 142: 1402-1406