Direct electrical quantification of glucose and asparagine from bodily fluids using nanopores.
Direct electrical quantification of glucose and asparagine from bodily fluids using nanopores.
复制标题
DOI：
10.1038/s41467-018-06534-1
复制
发表时间：
2018-10-05
影响因子：
16.6
通讯作者：
Maglia G
中科院分区：
文献类型：
--
作者：
Galenkamp NS;Soskine M;Hermans J;Wloka C;Maglia G
生物传感器的关键步骤是将生物信号转换为电流以及直接采样体液。在这里，我们表明，蛋白质传感器与纳米孔相结合，作为一个电换能器，可以准确地量化代谢物在真实的时间直接从纳升量的血液和其他体液。将纳米孔并入便携式电子设备将允许开发用于即时护理和家庭诊断的代谢物的灵敏、连续和非侵入性传感器。蛋白质纳米孔正在成为各种生物分子的传感器。在这里，作者开发了一种基于细菌毒素ClyA的纳米孔，结合葡萄糖和天冬酰胺的结合蛋白，以同时从各种未加工的稀释体液中检测这些生物分子。
Crucial steps in the miniaturisation of biosensors are the conversion of a biological signal into an electrical current as well as the direct sampling of bodily fluids. Here we show that protein sensors in combination with a nanopore, acting as an electrical transducer, can accurately quantify metabolites in real time directly from nanoliter amounts of blood and other bodily fluids. Incorporation of the nanopore into portable electronic devices will allow developing sensitive, continuous, and non-invasive sensors for metabolites for point-of-care and home diagnostics. Protein nanopores are emerging as sensors for a variety of biomolecules. Here the authors develop a nanopore based on the bacterial toxin ClyA, in conjunction with binding proteins for glucose and asparagine, to detect these biomolecules simultaneously from a variety of unprocessed, diluted body fluids.
登录
查看更多内容
影响因子：
3.4
作者：
Sutheran, Hannah Lise;Reynolds, Timothy
通讯作者：
Reynolds, Timothy
影响因子：
5.4
作者：
Tack, Cornelius;Pohlmeier, Harald;Pfuetzner, Andreas
通讯作者：
Pfuetzner, Andreas
DOI：
10.1016/j.bbrc.2013.07.111
发表时间：
2013-08-30
影响因子：
3.1
作者：
Khan, Faaizah;Pickup, John C.
通讯作者：
Pickup, John C.
影响因子：
16.8
作者：
Gouridis, Giorgos;Schuurman-Wolters, Gea K.;Poolman, Bert
通讯作者：
Poolman, Bert
影响因子：
3.8
作者：
Ge, XD;Tolosa, L;Rao, G
通讯作者：
Rao, G