DIRECTLY MEASURED DEFORMATION ENERGY OF PHOSPHOLIPID H-II HEXAGONAL PHASES
DIRECTLY MEASURED DEFORMATION ENERGY OF PHOSPHOLIPID H-II HEXAGONAL PHASES
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DOI：
10.1039/dc9868100029
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发表时间：
1986-01-01
影响因子：
3.4
通讯作者：
RAND, RP
中科院分区：
文献类型：
--
作者：
GRUNER, SM;PARSEGIAN, VA;RAND, RP
结合X射线衍射的渗透应力已被用来测量的能量的倒置六方（HII）相含有二油酰磷脂酰胆碱（DOPC）和乙醇胺（DOPE）。为了区分链堆积能与控制稳定性的其他因素的作用，比较了添加和不添加十四烷的能量。碳氢化合物的应变能存储在HII晶格中，但烷烃促进了从层状结构到HII结构的转变。在过量水的存在下，各种HII晶格寻求水-极性界面的自发曲率半径r 0。在低渗透应力下，从这个自发半径变形的工作似乎是二次的曲率，可以很好地描述由弯曲模量是独立的初始r 0，并与弯曲模量测量平面bilyaers。在高应力下，水的去除和晶格变形的工作似乎是从由脂质头基和水组成的极性“糊状物”中去除水，仅微弱地依赖于DOPE/DOPC比率。
Osmotic stress combined with X-ray diffraction has been used to measure the energies of inverted hexagonal (HII) phases containing dioleylphosphatidyl-choline (DOPC) and -ethanolamine (DOPE). To distinguish chain packing energies from the action of other factors governing stability, energies with and without added tetradecane have been compared. Little hydrocarbon strain energy is stored in the HII lattice, but alkane promotes the transition from a lamellar to an HII structure. In the presence of excess water, various HII lattices seek spontaneous radii of curvature, r0, of the aqueous–polar interface. Under low osmotic stress, the work of deforming from this spontaneous radius appears to be quadratic in curvature, can be well described by a bending modulus that is independent of the initial r0, and is related to bending moduli measured on planar bilyaers. At high stress, the work of water removal and lattice deformation seems to be that of removing water from a polar ‘mash’ composed of lipid headgroups and water, dependent only weakly on the DOPE/DOPC ratio.