fatvg encodes a new localized RNA that uses a 25-nucleotide element (FVLE1) to localize to the vegetal cortex of Xenopus oocytes.
fatvg encodes a new localized RNA that uses a 25-nucleotide element (FVLE1) to localize to the vegetal cortex of Xenopus oocytes.
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发表时间：
1999-11
期刊：
影响因子：
4.6
通讯作者：
A. P. Chan;M. Kloc;L. Etkin
中科院分区：
文献类型：
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作者：
A. P. Chan;M. Kloc;L. Etkin
植物定位转录本涉及许多重要的生物学功能，包括细胞命运决定和胚胎模式。我们已经分离出一个cDNA， fatvg，它编码一个局部的母体转录物，显示出一个类似于Vg1 mRNA的定位模式。Fatvg是一种在脂肪组织中表达的哺乳动物基因的同源物。与Vg1严格定位于晚期通路不同，fatvg转录本也与线粒体云相关，这是METRO或早期通路的特征。这表明，在卵子形成过程中，fatvg mRNA可能同时利用METRO和Late通路定位到植物皮层。我们解剖了fatvg mRNA的顺式作用定位元件，并将这些元件与Vg1 mRNA进行了比较。我们的研究结果表明，像大多数定位rna一样，在各种系统中，fatvg的转录本在3'UTR中包含定位元件。fatvg mRNA的3'UTR包含多个能够独立起作用的元件；然而，当所有元素都存在时，它的功能最有效。我们已经定义了一个短的25个核苷酸的元素，可以指导植物定位作为一个单一的拷贝。该元件与先前描述的Vg1定位元件的序列不同，表明不同的定位元件通过Late途径参与rna的定位。
Vegetally localized transcripts have been implicated in a number of important biological functions, including cell fate determination and embryonic patterning. We have isolated a cDNA, fatvg, which encodes a localized maternal transcript that exhibits a localization pattern reminiscent of Vg1 mRNA. fatvg is the homologue of a mammalian gene expressed in adipose tissues. The fatvg transcript, unlike Vg1 which localizes strictly through the Late pathway, also associates with the mitochondrial cloud that is characteristic of the METRO or Early pathway. This suggests that fatvg mRNA may utilize both the METRO and Late pathways to localize to the vegetal cortex during oogenesis. We have dissected the cis-acting localization elements of fatvg mRNA and compared these elements with Vg1 mRNA. Our results indicate that, like most localized RNAs, in a variety of systems, transcripts of fatvg contain localization elements in the 3'UTR. The 3'UTR of fatvg mRNA contains multiple elements that are able to function independently; however, it functions most efficiently when all of the elements are present. We have defined a short 25-nucleotide element that can direct vegetal localization as a single copy. This element differs in sequence from previously described Vg1 localization elements, suggesting that different localization elements are involved in the localization of RNAs through the Late pathway.