SYMBIOSIS BETWEEN IN-VIVO AND IN-VITRO NMR-SPECTROSCOPY - THE CREATINE, N-ACETYLASPARTATE, GLUTAMATE, AND GABA CONTENT OF THE EPILEPTIC HUMAN BRAIN
SYMBIOSIS BETWEEN IN-VIVO AND IN-VITRO NMR-SPECTROSCOPY - THE CREATINE, N-ACETYLASPARTATE, GLUTAMATE, AND GABA CONTENT OF THE EPILEPTIC HUMAN BRAIN
复制标题
DOI：
10.1016/0730-725x(95)02033-p
复制
发表时间：
1995-01-01
影响因子：
2.5
通讯作者：
SPENCER, DD
中科院分区：
文献类型：
--
作者：
PETROFF, OAC;PLEBAN, LA;SPENCER, DD
高分辨率的H-1核磁共振波谱分析颞叶活检获得癫痫患者。热稳定的大脑，透析的胞质大分子，高氯酸提取物进行了研究，使用一维和二维光谱。前颞叶新皮质富含GABA、谷氨酸、丙氨酸、N-乙酰天冬氨酸和肌酸。皮下白色物质富含天冬氨酸、谷氨酰胺和肌醇。N-乙酰天冬氨酸/肌酸摩尔比在前颞叶新皮层与内侧（0.66）低于新皮层（0.80）颞叶癫痫。使用密度梯度离心将人脑活检样品分离成粗品和精制的突触体、神经元细胞体和神经胶质。神经元组分富含谷氨酸和N-乙酰天冬氨酸。胶质细胞组分富含乳酸盐、谷氨酰胺和肌醇。癫痫皮质肌酸含量（8.8-8.9 mmol/kg）与正常枕叶肌酸含量（8.9 mmol/kg）相同。癫痫皮质谷氨酸含量（10.1-10.5 mmol/kg）高于正常枕叶（8.8 mmol/kg）。癫痫皮质GABA含量（2.3- 2.2mmol/kg）高于正常枕叶（1.2mmol/kg）。癫痫颞叶皮质N-乙酰天冬氨酸含量（5.8-6.8 mmol/kg）低于正常枕叶（8.9 mmol/kg）。成对的体内和体外测量对于明确理解癫痫患者H-1光谱的变化至关重要。
High resolution H-1 NMR spectroscopy was used to analyze temporal lobe biopsies obtained from patients with epilepsy. Heat-stabilized cerebrum, dialyzed cytosolic macromolecules, and perchloric acid extracts were studied using one- and two dimensional spectroscopy. Anterior temporal lobe neocortex was enriched in GABA, glutamate, alanine, N-acetylaspartate, and creatine. Subjacent white matter was enriched in aspartate, glutamine, and inositol. The N-acetylaspartate/creatine mole ratio was lower in anterior temporal neocortex with mesial (0.66) than neocortical (0.80) temporal lobe epilepsy. Human brain biopsy samples were separated into crude and refined synaptosomes, neuronal cell bodies, and glia using density gradient centrifugation. Neuronal fractions were enriched in glutamate and N-acetylaspartate. Glial cell fractions were enriched in lactate, glutamine, and inositol. The creatine content was the same in biopsied epileptic cortex (8.8-8.9 mmol/kg) and normal in vive occipital lobe (8.9 mmol/kg). Glutamate content was higher in epileptic cortex at biopsy (10.1-10.5 mmol/kg) than normal in vivo occipital lobe (8.8 mmol/kg). GABA content was higher in biopsies of epileptic cortex (2.3-2.2 mmol/kg) than in normal in vive occipital lobe (1.2 mmol/kg). N-acetylaspartate content was lower in biopsied epileptic temporal cortex (5.8-6.8 mmol/kg) than normal in vive occipital lobe (8.9 mmol/kg). Paired in vive and ex vive measurements are critical for a firm understanding of the changes seen in the H-1-spectra from patients with epilepsy.