Integrative analysis of multiple metabolomes and transcriptome revealed color expression mechanism in red skin root syndrome of Panax ginseng
Integrative analysis of multiple metabolomes and transcriptome revealed color expression mechanism in red skin root syndrome of Panax ginseng
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多代谢组和转录组综合分析揭示人参红皮根综合征颜色表达机制
DOI：
10.1016/j.indcrop.2021.114491
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发表时间：
2022-01-11
影响因子：
5.9
通讯作者：
You, Jiangfeng
中科院分区：
文献类型：
--
作者：
Luo, Ming;Li, Aixin;You, Jiangfeng
红皮根综合征导致人参产量和质量下降。然而，其发展过程和关键代谢产物仍然未知。对田间生长的红皮和健康人参进行了多种代谢组学研究，包括非靶向代谢组学研究、花青素靶向代谢组学研究和类胡萝卜素靶向代谢组学研究。结合先前的转录组结果分析检测到的代谢物。总代谢产物谱显示，红皮参中初级代谢产物尤其是柠檬酸盐含量较健康参有所减少，而主要参与苯丙素途径的次级代谢产物含量较健康参有所增加。靶向代谢组学发现，每克新鲜红皮人参样品含有8微克β-胡萝卜素，0.56微克α-胡萝卜素，0.017微克芦丁，0.248微克查耳酮，以及一些花青素，包括0.023微克矢车菊素和0.037微克槲皮素3-O-葡萄糖苷，尽管浓度较低，但高于健康人参。此外，整合代谢组学和转录组学分析表明，与花青素合成相关的代谢产物的变化与相关基因的表达模式相关。此外，茉莉酸（JA）及其前体12-氧代植物二烯酸（12-oxophytodienoic acid）在红皮人参中的浓度显著增加，表明JA或JA信号通路在调节红皮综合征中可能发挥作用。因此，17个MYB转录因子在红皮参中的表达存在差异。JA相关的顺式元件广泛分布在其启动子上。综上所述，我们的研究揭示了红皮人参的初级代谢减少，但次级代谢增加。JA信号通路中的MYB转录因子可能介导了红皮人参花色素苷和类胡萝卜素的积累，从而促进红皮人参的发育。
Red skin root syndrome causes reduction of both production and quality of Panax ginseng (ginseng). However, its development process and key metabolites are still unknown. Multiple metabolomes including non-targeted metabolome, and anthocyanins-targeted and carotenoids-targeted metabolomes were performed on field grown red skin and healthy ginseng. The detected metabolites were analyzed in combination with previous transcriptome results. The total metabolite profiles revealed that primary metabolites especially citrate was decreased, but secondary metabolites mainly involved in phenylpropanoid pathway were increased in red skin ginseng compared to those in healthy ginseng. Targeted metabolome found that each gram of fresh red skin ginseng sample contained 8 mu g beta-carotene, 0.56 mu g alpha-carotene, 0.017 mu g rutin, 0.248 mu g chalcone, and some anthocyanins including 0.023 mu g cyanidin and 0.037 mu g quercetin 3-O-glucoside, albeit in low concentrations but higher than those in healthy ginseng. Furthermore, integrative metabolome and transcriptome analysis revealed that changes of metabolites related to anthocyanins synthesis were correlated with the related gene expression patterns. Additionally, jasmonate (JA) and its precursor 12-oxophytodienoic acid increased their concentrations dramatically in red skin ginseng, suggesting the possible role of JA or JA signaling pathway in regulating red skin syndrome. Consistently, the expressions of 17 MYB transcription factors were differentially regulated in red skin ginseng. JA related cis-elements are widely distributed at their promoters. Taken together, our study revealed reduction of primary metabolism but increase of secondary metabolism in red skin ginseng. MYB transcription factors of JA signal pathway were supposed to mediate accumulation of anthocyanins and carotenoids that contributed to the development of red skin ginseng.