Isolation and characterization of four novel thraustochytrid strains from a colonial tunicate
Isolation and characterization of four novel thraustochytrid strains from a colonial tunicate
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从殖民地被囊动物中分离和表征四种新型破囊壶菌菌株
DOI：
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
B. Rinkevich
中科院分区：
文献类型：
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作者：
C. Rabinowitz;J. Douek;R. Weisz;A. Shabtay;B. Rinkevich
Thraustochytrids是一类常见的海洋真核原生生物，是无脊椎原代细胞培养的主要污染物。在这里，我们试图从殖民地被囊植物Botryllus schlosseri的拔芽原代培养物中分离出thraustochytrid物种。分离到4株新菌株，命名为BS3-BS6。这些菌株的细胞在三种培养基条件下表现出不同的生长模式和细胞形态。系统发育（18S rDNA）和AFLP分析显示菌株BS3和BS5和BS4和BS6具有两种特征。序列分析表明，菌株BS3与BS5的同源性为98%，菌株BS4与BS6的同源性为100%。距离分析表明，这4个新菌株与几年前从同一种被囊动物中分离出来的另外2个菌株有亲缘关系。结果表明，从schlosbotryllus中分离到的6株亲缘关系较近的水蛭系菌株，在分子标记、生长模式和细胞特征等方面，揭示了水蛭系与水蛭种/隐种分支之间的生物学联系。
Thraustochytrids, a common group of marine eukaryotic protists, are the major contaminants of invertebrate primary cell cultures. Here we have attempted to isolate thraustochytrid species from primary cultures of extirpated buds of the colonial tunicate Botryllus schlosseri. Four new strains (termed BS3-BS6) were isolated. Cells of these strains subjected to three media conditions, showed different growth patterns and cellular morphologies. Phylogenetic (18S rDNA) and AFLP analyses revealed two-band patterns, one characteristic to strains BS3 and BS5 and the other to strains BS4 and BS6. A Sequence analysis showed 98 % identity between strains BS3 and BS5, and 100 % identity between strains BS4 and BS6. Distance analysis tree showed that the four new strains were primarily related to two other thraustochytrids strains, independently isolated from the same tunicate species a few years ago. It is suggested that the six closely related thraustochytrid strains isolated from Botryllus schlosseri reveal a biological association between the tunicate and a clade of species/cryptic species of thraustochytrids, which can be identified by molecular markers, growth patterns and cellular characterizations.
灰霉病菌苍白芽的上皮细胞培养：成就和挑战。
DOI：
10.1007/s11022-004-2087-9
发表时间：
2003
期刊：
Methods in cell science : an official journal of the Society for In Vitro Biology.
影响因子：
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作者：
Rabinowitz,Claudette;Rinkevich,Baruch
通讯作者：
Rinkevich,Baruch
硬壳蛤 Mercenaria Mercenaria 的原生生物寄生虫 QPX 是迷宫菌门 (Labyrinthulomycota) 的成员。
影响因子：
1.4
作者：
Ragan,MA;MacCallum,GS;Murphy,CA;Cannone,JJ;Gutell,RR;McGladdery,SE
通讯作者：
McGladdery,SE