Simultaneous infrared thermal imaging and laser speckle imaging of brain temperature and cerebral blood flow in rats
Simultaneous infrared thermal imaging and laser speckle imaging of brain temperature and cerebral blood flow in rats
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DOI：
10.1117/1.jbo.24.3.031014
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发表时间：
2019-03-01
影响因子：
3.5
通讯作者：
Fukuyama, Hidenao
中科院分区：
文献类型：
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作者：
Suzuki, Takashi;Oishi, Naoya;Fukuyama, Hidenao
大脑温度变化的红外热成像可用于评估皮质活动和疾病状态，例如中风。然而，这些变化取决于新陈代谢产生的热量变化和与血流相关的热对流变化之间的平衡。为了区分这些影响并更清楚地了解神经血管代谢耦合，必须改进脑温度成像以同时测量温度和血流量。我们开发了一种成像技术，通过将红外热成像与激光散斑成像相结合，显示麻醉大鼠绝对脑温度和相对脑血流变化的二维 (2-D) 分布。使用两种不同的麻醉剂（异氟烷和α-氯醛糖）实现大脑代谢和脑血流量的变化来评估我们的系统。异氟烷增加脑血流量，但降低新陈代谢，而α-氯醛糖则降低这两个参数。该技术能够同时可视化同一区域的大脑表面温度和脑血流量的变化。该成像系统将允许进一步研究正常和患病大脑中的神经血管代谢耦合。 (C) 作者。由 SPIE 根据 Creative Commons Attribution 3.0 Unported 许可证发布。
Infrared thermal imaging of brain temperature changes is useful for evaluating cortical activity and disease states, such as stroke. However, the changes depend on a balance between changes in heat generation from metabolism and in heat convection related to blood flow. To discriminate between these effects and gain a clearer understanding of neurovascular metabolic coupling, brain temperature imaging must be improved to measure temperature and blood flow simultaneously. We develop an imaging technique that shows a two-dimensional (2-D) distribution of absolute brain temperature and relative cerebral blood flow changes in anesthetized rats by combining infrared thermal imaging with laser speckle imaging. The changes in brain metabolism and cerebral blood flow are achieved using two different anesthetics (isoflurane and alpha-chloralose) to evaluate our system. Isoflurane increased cerebral blood flow but decreased metabolism, whereas alpha-chloralose decreased both parameters. This technique enables simultaneous visualization of brain surface changes in temperature and cerebral blood flow in the same regions. This imaging system will permit further study of neurovascular metabolic coupling in normal and diseased brains. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.