Qualitative and quantitative analyses of the bifidobacterial microbiota in the colonic mucosa of patients with colorectal cancer, diverticulitis and inflammatory bowel disease
Qualitative and quantitative analyses of the bifidobacterial microbiota in the colonic mucosa of patients with colorectal cancer, diverticulitis and inflammatory bowel disease
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DOI：
10.3748/wjg.v13.i29.3985
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发表时间：
2007-08-07
影响因子：
4.3
通讯作者：
Salminen, Seppo
中科院分区：
文献类型：
--
作者：
Gueimonde, Miguel;Ouwehand, Arthur;Salminen, Seppo
目的：了解结肠癌、炎症性肠病和憩室炎患者结肠黏膜双歧杆菌菌群特征。方法：手术中采集34例患者的结肠远端粘膜样本，其中21例诊断为结直肠癌，9例诊断为憩室炎，4例诊断为炎性肠病，需要手术治疗。从切除的粘膜样品中提取细菌DNA，采用定性和定量PCR方法对双歧杆菌粘膜相关菌群进行定性和定量测定。结果：100%的憩室炎或IBD患者样本中发现双歧杆菌，76%的结肠癌患者样本中发现双歧杆菌。其中，长白僵菌和两歧白僵菌分布最广，其次为动物白僵菌、连环白僵菌和青春期白僵菌。所有样品均未检出短螺旋体、牙螺旋体和角螺旋体。憩室炎患者长梭菌的发生率明显高于结肠癌和IBD患者（分别为100%、62%和75%，P < 0.05）。在动物B.（56%， 0%和25%，P < 0.05）中也发现了类似的结果，而B.青春期B.仅在结肠癌患者的粘膜中发现（5 / 21,24%）。在定量水平上，结肠癌和IBD患者的双歧杆菌总数（分别为4.94和5.91对数细胞/样本，P < 0.05）和长芽孢杆菌总数（分别为4.05和4.79对数细胞/样本，P < 0.05）低于憩室炎患者。结论：粘膜相关菌群的异常存在于不同的肠道疾病中。这可能表明微生物群在这些疾病的发病机制中的作用。(c) 2007年WJG。版权所有。
AIM: To characterize the bifidobacterial microbiota of the colonic mucosa in patients with colon cancer, inflammatory bowel disease or diverticulitis.METHODS: A sample of the distal colonic mucosa was taken during surgery from a total of 34 patients, twenty-one with diagnosed colorectal cancer, nine with diverticulitis and four with inflammatory bowel disease, requiring surgery for their condition. Bacterial DNA was extracted from the resected mucosal samples and bifidobacterial mucosa-associated microbiota was qualitatively and quantitatively determined by means of qualitative and quantitative PCR.RESULTS: Bifidobacteria were found in 100% of the samples from patients with diverticulitis or IBD and a 76% of those suffering colon cancer. The species B. longum and B. bifidum were the most widely found, followed by B. animalis, B. catenulatum and B. adolescentis. B. breve, B. dentium and B. angulatum were not detected in any sample. A significantly higher occurrence of B. longum was observed in patients with diverticulitis than in those with colon cancer or IBD (100%, 62% and 75%, respectively, P < 0.05). Similar results were obtained for B. animalis (56%, 0% and 25%, P < 0.05), while B. adolescentis was only found in the mucosa from patients with colon cancer (5 out of 21, 24%). At the quantitative level, patients with colon cancer or IBD showed lower counts of total Bifidobacterium (4.94 and 5.91 vs 6.96 log Cells/sample, respectively, P < 0.05) and of the species B. longum (4.05 and 4.79 vs 6.76, P < 0.05) than those with diverticulitis.CONCLUSION: Aberrancies in mucosa associated microbiota are present in different intestinal diseases. This may indicate a role of the microbiota in the pathogenesis of these diseases. (c) 2007 WJG. All rights reserved.