A new method for SNP discovery.
A new method for SNP discovery.
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SNP 发现的新方法。
DOI：
10.2144/000113075
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发表时间：
2009
期刊：
影响因子：
2.7
通讯作者：
Song‐lin Chen
中科院分区：
文献类型：
--
作者：
Jian;Gen;Song‐lin Chen
单核苷酸多态性（snp）是基因组中高密度的自然序列变异。它们被认为是特定物种内表型变异的主要遗传来源，并作为优良的遗传标记。snp在鉴定导致疾病和表型性状的候选基因方面是有用的。在非模式生物中，由于目前可用的SNP分离技术所涉及的费用和技术困难，SNP的应用受到限制。在本研究中，我们开发了一种快速有效的方法来随机分离整个基因组的snp。含有snp的DNA片段可以从背景DNA中高效分离出来。我们用这种方法分析了中国新开发的具有重要商业价值的半光滑舌鳎（Cynoglossus semilaevis）的10个分离DNA片段，发现其中9个片段含有snp。这些发现在不同的个体中得到了成功的证实。这里提出的方法具有成本效益，基本上适用于任何生物体。
Single nucleotide polymorphisms (SNPs) are high-density natural sequence variations in genomes. They are considered to be the major genetic source of phenotypic variability within a given species and serve as excellent genetic markers. SNPs are useful in identifying candidate genes that contribute to disease and phenotypic traits. In non-model organisms, the application of SNPs has been limited, because of the expense and technical difficulties entailed in currently available SNP isolation techniques. In the present study, we have developed a rapid and effective method to isolate SNPs throughout the genome randomly. The DNA fragments containing SNPs could be isolated efficiently from background DNA. We analyzed ten isolated DNA fragments with this method in half-smooth tongue sole (Cynoglossus semilaevis)--a newly exploited and commercially important cultured marine flatfish in China--and found that nine of the fragments contained SNPs. The findings were confirmed successfully in different individuals. The method presented here is cost-effective and applicable to essentially any organism.
影响因子：
9.3
作者：
Gundry, CN;Vandersteen, JG;Wittwer, CT
通讯作者：
Wittwer, CT
影响因子：
14.9
作者：
Oleykowski, CA;Mullins, CRB;Yeung, AT
通讯作者：
Yeung, AT
影响因子：
7
作者：
Borevitz, JO;Liang, D;Chory, J
通讯作者：
Chory, J