Single-step detection of norovirus tuning localized surface plasmon resonance-induced optical signal between gold nanoparticles and quantum dots
Single-step detection of norovirus tuning localized surface plasmon resonance-induced optical signal between gold nanoparticles and quantum dots
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DOI：
10.1016/j.bios.2018.09.024
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发表时间：
2018-12-30
影响因子：
12.6
通讯作者：
Park, Enoch Y.
中科院分区：
文献类型：
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作者：
Nasrin, Fahmida;Chowdhury, Ankan Dutta;Park, Enoch Y.
利用金纳米粒子(AuNPs)和荧光量子点(QD)的局域表面等离子体共振(LSPR)行为，提出了一种对病毒无标记具有良好选择性和灵敏度的无标记传感新方法。通过配体交换反应，用(L)-半胱氨酸修饰了无机四元合金的CdSeTeS量子点。或者，柠檬酸稳定的金纳米粒子被11-巯基十一酸功能化，以在金表面生成羧基。将修饰在AuNPs表面的羧基与(L)-半胱氨酸修饰的CdSeTeS量子点的胺基团共价键合，形成了CdSeTeS量子点/AuNPs纳米复合材料。荧光光谱显示，由于与AuNPs的紧密相互作用，在640 nm处，CdSeTeS量子点发生猝灭。然而，在连续加入诺如病毒样颗粒(NOV-LP)后，空间位阻诱导的来自相邻AuNPs的LSPR信号触发了量子点的荧光增强，其程度与靶NOV-LP的浓度成比例。线性范围为10(-14)~10(-9)g mL(-1)，检出限为12.1×10(-15)g mL(-1)。将该方法应用于临床分离的诺如病毒的检测，检测范围为10(2)-10(5)拷贝(-1)，检测下限为95.0拷贝(-1)，比市售试剂盒高100倍。与其他传统传感器相比，提出的传感器的优势在于即使在临床分离的样本中也能在低病毒浓度下进行超灵敏检测。该检测方法为生物医学应用中的病毒检测提供了一种高性能、高稳定性的传感探针。
A new method of label free sensing approach with superior selectivity and sensitivity towards virlabel-freeon is presented here, employing the localized surface plasmon resonance (LSPR) behavior of gold nanoparticles (AuNPs) and fluorescent CdSeTeS quantum dots (QDs). Inorganic quaternary alloyed CdSeTeS QDs were capped with (L)-cysteine via a ligand exchange reaction. Alternatively, citrate stabilized AuNPs were functionalized with 11-mercaptoundecanoic acid to generate carboxylic group on the gold surface. The carboxylic group on the AuNPs was subjected to bind covalently with the amine group of (L)-cysteine capped CdSeTeS QDs to form CdSeTeS QDs/AuNPs nanocomposites. The fluorescence of CdSeTeS QDs/AuNPs nanocomposite shows quenched spectrum of CdSeTeS QDs at 640 nm due to the close interaction with AuNPs. However, after successive addition of norovirus-like particles (NoV-LPs), steric hindrance-induced LSPR signal from the adjacent AuNPs triggered the fluorescence enhancement of QDs in proportion to the concentration of the target NoV-LPs. A linear range of 10(-14) to 10(-9) g mL(-1) NoV-LPs with a detection limit of 12.1 x 10(-15) g mL(-1) was obtained. This method was further applied on clinically isolated norovirus detection, in the range of 10(2)-10(5) copies mL(-1) with a detection limit of 95.0 copies mL(-1), which is 100-fold higher than commercial ELISA kit. The superiority of the proposed sensor over other conventional sensors is found in its ultrasensitive detectability at low virus concentration even in clinically isolated samples. This proposed detection method can pave an avenue for the development of high performance and robust sensing probes for detection of virus in biomedical applications.