Real-time polymerase chain reaction quantification of Epstein-Barr virus in chronic periodontitis patients
Real-time polymerase chain reaction quantification of Epstein-Barr virus in chronic periodontitis patients
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DOI：
10.1111/j.1600-0765.2005.00796.x
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发表时间：
2005-08-01
影响因子：
3.5
通讯作者：
Antoniadis, A
中科院分区：
文献类型：
--
作者：
Konstantinidis, A;Sakellari, D;Antoniadis, A
背景资料：虽然疱疹病毒与慢性和侵袭性牙周炎的发病机制有关，但文献中很少有数据涉及牙周部位这些病毒的定量，特别是与血清学结果有关。目的：本研究的目的是比较慢性牙周炎患者和牙周健康受试者龈下标本中EB病毒（EBV）DNA载量，方法：对22例慢性牙周炎患者和13例健康对照者进行血清学EBV IgM和IgG抗体检测。79龈下标本（一个合并，一个从深，一个从浅网站），采样纸点，进行了分析与EBV的实时聚合酶链反应。受试者还检查了血清中的抗EBV IgG和IgM水平，使用酶联免疫吸附试验。3例受试者（1例患者和2例对照）显示抗EBV IgM。他们的数据被排除在进一步分析之外。所有三个显示在其龈下样本EBV。其余20名慢性牙周炎患者中有9名和11名对照中有10名未显示牙龈下EBV。一个统计学上显着的差异，在合并和浅袋样本牙周炎患者之间观察到的病毒载量，但不是从深和浅袋样本之间（Kruskall-Wallis方差分析，邓恩的多重比较检验）。结论：本研究的数据不强烈支持慢性牙周炎病变的EBV的发病意义。然而，这些数据确实表明，平行的血清学评估提供了一个有用的洞察与牙周病病毒的关联。
Background: Although herpes viruses have been implicated in the pathogenesis of chronic and aggressive periodontitis, few data in the literature refer to quantification of these viruses in periodontal sites, especially in relation to serological findings.Objective: The aim of the present study was to compare Epstein-Barr virus (EBV) DNA load in subgingival specimens from chronic periodontitis patients and in periodontally healthy subjects, in relation to serologic testing of IgM and IgG antibodies to EBV.Methods: A total of 22 chronic periodontitis patients and 13 controls participated in the present study. Seventy-nine subgingival specimens (one pooled, one from a deep and one from a shallow site), sampled with paper points, were analysed with real-time polymerase chain reaction for EBV. Subjects were also examined for anti-EBV IgG and IgM levels in serum, using an enzyme-linked immunosorbent assay.Results: One subject was seronegative for EBV. Three subjects (one patient and two controls) displayed anti-EBV IgM. Their data were excluded from further analysis. All three displayed EBV in their subgingival samples. Nine out of the remaining 20 chronic periodontitis patients and 10 out of 11 controls did not display EBV subgingivally. A statistically significant difference in viral load was observed between pooled and shallow-pocket samples from periodontitis patients but not between samples from deep and shallow pockets (Kruskall-Wallis ANOVA, Dunn's multiple comparisons test).Conclusions: Data from the present study do not strongly support the pathogenetic significance of EBV in chronic periodontitis lesions. The data do, however, suggest that parallel serological assessments provide a useful insight into the association of viruses with periodontal disease.