Role of cytokines in lipopolysaccharide-induced functional and structural abnormalities of astrocytes.
Role of cytokines in lipopolysaccharide-induced functional and structural abnormalities of astrocytes.
复制标题
细胞因子在脂多糖诱导的星形胶质细胞功能和结构异常中的作用。
作者：
Hu,S;Martella,A;Anderson,WR;Chao,CC
脑膜炎相关脑损伤的潜在机制尚不清楚。本研究探讨了脂多糖（LPS）通过释放促炎细胞因子改变星形胶质细胞功能和结构的假设。在富集的小鼠星形胶质细胞培养物中，LPS抑制（P < 0.05）谷氨酰胺合成酶活性、3 H-γ氨基丁酸摄取和DNA合成; LPS还诱导超微结构变化。肿瘤坏死因子-α、白细胞介素-1和白细胞介素-6抗体部分阻断了LPS诱导的星形胶质细胞功能抑制（P < 0.05）。此外，用细胞因子处理星形胶质细胞培养物显著改变了这些星形胶质细胞的功能和超微结构。总之，目前的研究结果支持LPS通过释放促炎细胞因子，特别是肿瘤坏死因子-α影响星形胶质细胞功能和结构的假设。© 1994 Wiley利斯公司
The mechanism underlying meningitis‐associated brain injury is unclear. This study investigated the hypothesis that lipopolysaccharide (LPS) alters astrocyte function and structure via the release of proinflammatory cytokines. In enriched murine astrocyte cultures, LPS inhibited (P < 0.05) glutamine synthetase activity,3H‐gamma aminobutyric acid uptake, and DNA synthesis; LPS also induced ultrastructural changes. Antibodies to tumor necrosis factor‐α, interleukin‐1, and interleukin‐6 blocked (P < 0.05) in part the LPS‐induced inhibition of astrocyte function. Also, treatment of astrocyte cultures with cytokines significantly altered these astrocyte functions and ultrastructure. Taken together, the present findings support the hypothesis that LPS affects astrocyte function and structure via the release of proinflammatory cytokines, especially tumor necrosis factor‐α. © 1994 Wiley‐Liss, Inc.