Effects of dietary supplemental methionine source and betaine replacement on the growth performance and activity of mitochondrial respiratory chain enzymes in normal and heat-stressed broiler chickens
Effects of dietary supplemental methionine source and betaine replacement on the growth performance and activity of mitochondrial respiratory chain enzymes in normal and heat-stressed broiler chickens
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DOI：
10.1111/jpn.13005
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发表时间：
2019-01-01
影响因子：
2.7
通讯作者：
Golian, Abolghasem
中科院分区：
文献类型：
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作者：
Ala, Fatemeh Sahebi;Hassanabadi, Ahmad;Golian, Abolghasem
本试验旨在评价饲粮中添加蛋氨酸源和甜菜碱替代蛋氨酸对正常和热应激肉鸡生产性能和线粒体呼吸链酶活性的影响。试验选用1200日龄罗斯308鸡，分为2个鸡舍，每个鸡舍12个处理，5个重复，每个重复10只鸡，每个重复采用2 × 2x3 × 2（分别为温度× Met源× Met水平× Bet）分样析因试验。基础饲粮中的蛋氨酸水平为需取量的70%，通过补充dl-或l-Met提高到需取量的130%。以补充30% dl-或l-Met的速率替换Bet或不替换Bet。1 ~ 10 d， 70% l-蛋氨酸饲粮的饲料系数（FCR）低于70% dl-蛋氨酸饲粮（p = 0.04）。饲粮蛋氨酸水平为130%或130%的肉鸡在11-42 d内的增重均高于饲粮蛋氨酸水平为70%的肉鸡（p < 0.001）。饲粮中蛋氨酸含量为130%的肉仔鸡在11 ~ 42 d的采食量显著低于其他2组（p < 0.05）。饲粮中蛋氨酸需取量为130%的组在11 ~ 42 d的饲料效率低于其他两组（p < 0.001）。热应激鸡的生长速度低于正常条件下（p < 0.05）。常温条件下，Req Met饲粮的肉仔鸡Cox I和III活性显著提高（p < 0.05）。在常温下，Bet +日粮与Bet-日粮的热应激鸟的Cox I活性相似（p = 0.046）。综上所述，随着蛋氨酸水平的升高，蛋鸡的生产性能和Cox I、Cox III活性均有所提高。与不添加蛋氨酸的饲粮相比，添加30%的蛋氨酸对生产性能的影响相似，但增加了Cox III的活性。在细胞水平上，l-Met比dl-Met更有效。高环境温度抑制性能和MRCE活性。
This study aimed to evaluate the effects of dietary supplemental methionine (Met) source and betaine (Bet) replacement for Met on performance and activity of mitochondrial respiratory chain enzymes (MRCEs) in normal and heat-stressed broiler chickens. Total of 1,200-day-old Ross 308 chicks were allocated to two houses, each consisted of 12 treatments, five replicates of 10 birds each with 2 x 2x3 x 2 (temperature x Met source x Met level x Bet, respectively) split-plot factorial arrangement. Met level in the basal diets was 70% requirements (Req) that was increased to the requirement or 130% by supplemental dl- or l-Met. Bet was or was not substituted at the rate of 30% supplemental dl- or l-Met. Feed conversion ratio (FCR) in chicks fed 70% l-Met was lower than those fed 70% dl-Met diet during 1-10 days (p = 0.04). Broilers fed diets containing requirement or 130% Met, regardless of its source, showed higher weight gain (WG) than those received 70% Met diet during 11-42 days (p < 0.001). Feed intake (FI) of broilers fed 130% Met diet was decreased compared to other two groups during 11-42 days (p < 0.05). One hundred thirty percent Met requirement diet resulted in lower FCR comparing to other two groups during 11-42 days (p < 0.001). Heat-stressed birds grew less than those under normal condition (p < 0.05). Broilers fed Req Met diet under normal temperature exhibited higher activities of complexes (Cox) I and III (p < 0.05). Cox I activity in heat-stressed birds fed Bet + diet was similar to those fed Bet-diet under normal temperature (p = 0.046). It is concluded that performance and the activities of Cox I and III were increased as the level of Met increased. Bet replacement for 30% supplemental Met resulted in similar consequences comparing to non-Bet replacement diets on performance, but increased the activity of Cox III. l-Met was effective than dl-Met at the cellular level. High ambient temperature depressed performance and MRCE activity.