Sequencing, Functional Annotation, and Interaction Prediction of mRNAs and Candidate Long Noncoding RNAs Originating from Tea Leaves During Infection by the Fungal Pathogen Causing Tea Leaf Spot, Didymella bellidis.
Sequencing, Functional Annotation, and Interaction Prediction of mRNAs and Candidate Long Noncoding RNAs Originating from Tea Leaves During Infection by the Fungal Pathogen Causing Tea Leaf Spot, Didymella bellidis.
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DOI：
10.1094/pdis-05-22-1240-a
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发表时间：
2023-09
期刊：
影响因子：
4.5
通讯作者：
Honglin Huang;Hongke Huang;Zhong-Min Xia;Yuqin Yang;Xinyue Jiang;Chen-Chia Huang;Yuanyou Yang;Delu Wang;Zhuo Chen
中科院分区：
文献类型：
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作者：
Honglin Huang;Hongke Huang;Zhong-Min Xia;Yuqin Yang;Xinyue Jiang;Chen-Chia Huang;Yuanyou Yang;Delu Wang;Zhuo Chen
茶树褐斑病可严重影响茶叶的产量和品质。中国西南部贵州省的叶。分析茶树的信使RNA（mRNAs）和长链非编码RNA（lncRNAs）之间的关系可以为植物与病原菌的相互作用提供新的认识。本研究利用高通量测序技术对茶尺蠖侵染过程中茶叶中的mRNA和lncRNA进行了分析。使用Illumina Novaseq 6000平台进行bellidis。D.感染bellidis菌丝分别导致553和191个差异表达的mRNA（DEmRNA）上调或下调。作为S基因数（在指定的Gene Ontology [GO]数据库中注释的具有显著差异表达的基因的总数），在生物学过程水平上相对于对真菌的防御应答富集了3个。在D. bellidis菌丝，而LRR受体样丝氨酸/苏氨酸蛋白激酶ERECTA（TEA016781.1）基因的表达下调。D. bellidis菌丝导致227和958个差异表达的lncRNA（DElncRNA）上调或下调。与未表征的LOC 101499401相关的DEmRNA（TEA015626.1），未表征蛋白质（TEA014125.1），染色体结构维持蛋白1（TEA001660.1）和未表征蛋白质20036、MSTRG. 3843、MSTRG. 26132和MSTRG. 56701的顺式调节的结果。利用基因表达谱分析技术和lncRNA/mRNA关联预测技术，研究了茶树叶片感染茶斑病菌后的表达谱和lncRNA/mRNA关联预测。bellidis将为进一步研究抗病性提供宝贵的资源。
Tea leaf spot caused by Didymella bellidis can seriously reduce the productivity and quality of tea (Camellia sinensis var. sinensis) leaves in Guizhou Province, southwest China. Analysis of the relationship between messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs) of tea could provide insights into the plant-pathogen interaction. In this study, high-throughput sequencing of mRNAs and lncRNAs from tea leaves during infection by D. bellidis was conducted using the Illumina Novaseq 6000 platform. Infection by D. bellidis hyphae resulted in up- or downregulation of 553 and 191 of the differentially expressed mRNAs (DEmRNAs), respectively. As the S gene number (total number of genes with significantly differential expression annotated in the specified Gene Ontology [GO] database), three were enriched with respect to the defense response to the fungus at the biological process level. Expression of the DEmRNAs peroxidase 21 (TEA000222.1) and mcht-2 (TEA013240.1) originating from tea leaves were upregulated during challenge by D. bellidis hyphae, whereas expression of the LRR receptor-like serine/threonine-protein kinase ERECTA (TEA016781.1) gene was downregulated. The infection of D. bellidis hyphae resulted in up- or downregulation of 227 and 958 of the differentially expressed lncRNAs (DElncRNAs). The DEmRNAs associated with uncharacterized LOC101499401 (TEA015626.1), uncharacterized protein (TEA014125.1), structural maintenance of chromosomes protein 1 (TEA001660.1), and uncharacterized protein (TEA017727.1) occurred as a result of cis regulation by DElncRNAs MSTRG.20036, MSTRG.3843, MSTRG.26132, and MSTRG.56701, respectively. The expression profiling and lncRNA/mRNA association prediction in the tea leaves infected by D. bellidis will provide a valuable resource for further research into disease resistance.