One-Eyed Pinhead and Spadetail are essential for heart and somite formation
One-Eyed Pinhead and Spadetail are essential for heart and somite formation
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DOI：
10.1038/ncb862
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发表时间：
2002-10-01
影响因子：
21.3
通讯作者：
Kimelman, D
中科院分区：
文献类型：
--
作者：
Griffin, KJP;Kimelman, D
对斑马鱼斑马鱼的突变分析表明，T 盒转录因子 Spadetail (Spt)(1) 和节点受体辅助因子 One-Eyed Pinhead (Oep)(2) 在中胚层和内胚层形成中具有独特的发育作用。在这里，我们表明 spt 和 oep 在遗传上相互作用，并且对于心脏和体节中胚层的形成至关重要。这两种中胚层缺陷取决于节点信号传导的不同效应器；心脏中胚层的形成涉及混合样转录因子 Bonnie 和 Clyde (Bon)(3)，而体节发生则依赖于不同的途径。 Zoep体节缺陷分析； spt 胚胎为了解 Spt 对体细胞中胚层形成的控制提供了见解，此前该研究涉及细胞粘附和运动的调节 (4,5)。我们证明了 Zoep 中体节的形成失败； spt胚胎与此无关，并且Spt必须具有额外的功能。我们认为Spt在体细胞发生中的主要作用是通过拮抗祖细胞型基因表达和行为来促进体节前中胚层与尾芽祖细胞的分化。
Mutant analysis in the zebrafish Danio rerio has demonstrated distinct developmental roles for the T-box transcription factor Spadetail (Spt)(1) and the Nodal-receptor cofactor One-Eyed Pinhead (Oep)(2) in the formation of mesoderm and endoderm. Here, we show that spt and oep genetically interact and are together essential for the formation of cardiac and somitic mesoderm. These two mesodermal defects are dependent on different effectors of Nodal signalling; cardiac mesoderm formation involves the mix-like transcription factor Bonnie and Clyde (Bon)(3), whereas somitogenesis is dependent on a different pathway. Analysis of the somite defect in Zoep; spt embryos has provided insights into the control of somitic mesoderm formation by Spt, which was previously implicated in the regulation of cell adhesion and motility(4,5). We show that the failure to form somites in Zoep; spt embryos is independent of this and that Spt must have an additional function. We propose that the major role of Spt in somitogenesis is to promote the differentiation of presomitic mesoderm from tailbud progenitors by antagonizing progenitor-type gene expression and behaviour.