Neuroprotective Properties of Panax notoginseng Saponins via Preventing Oxidative Stress Injury in SAMP8 Mice
Neuroprotective Properties of Panax notoginseng Saponins via Preventing Oxidative Stress Injury in SAMP8 Mice
复制标题
三七皂苷通过预防 SAMP8 小鼠氧化应激损伤的神经保护特性
DOI：
10.1155/2017/8713561
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发表时间：
2017-01-01
影响因子：
--
通讯作者：
Zhong, Zhen-Guo
中科院分区：
文献类型：
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作者：
Huang, Jin-Lan;Jing, Xin;Zhong, Zhen-Guo
抑制早期阿尔茨海默病（AD）的氧化损伤被认为是治疗AD的一种策略。我们的前期研究表明，三七皂苷（PNS）通过提高衰老大鼠血清中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-PX）水平而具有抗衰老作用。在本研究中，我们旨在研究PNS对AD小鼠氧化应激中抗氧化酶和解偶联蛋白（UCPs）的影响。结果表明，PNS可防止海马CA1区神经元丢失，减轻CA1区神经元的病理形态学改变。此外，PNS抑制8-羟基脱氧鸟苷（8-OHdG）的产生，增强SOD、CAT、GSH-PX的表达和活性，提高SAMP8小鼠脑内UCP4、UCP5 mRNA和蛋白水平。综上所述，我们的研究表明，PNS通过减少8-OHdG的产生，增强抗氧化酶的活性以及UCP4和UCP5的表达水平，具有保护AD脑神经元免受氧化应激损伤的能力。因此，PNS可能是一种很有前途的治疗AD的药物。
Inhibiting oxidative damage in early stage of Alzheimer's disease (AD) is considered as a strategy for AD treatment. Our previous study has shown that Panax notoginseng saponins (PNS) have an antiaging action by increasing the levels of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX) in the serum of aged rats. In this study, we aimed to investigate the effects of PNS on antioxidant enzymes and uncoupling proteins (UCPs) involved in oxidative stress in AD mice. The results showed that PNS prevented neuronal loss in hippocampal CA1 region and alleviated pathomorphological change of neurons in CA1 region. Moreover, PNS inhibited the production of 8-hydroxydeoxyguanosine (8-OHdG), enhanced the expressions and activities of SOD, CAT, and GSH-PX, and improved the mRNA and protein levels of UCP4 and UCP5 in the brains of SAMP8 mice. Together, our study shows that PNS has the ability to protect neurons in AD brain from oxidative stress damage through attenuating the production of 8-OHdG, enhancing the activities of antioxidant enzymes and the expressions levels of UCP4 and UCP5. Accordingly, PNS may be a promising agent for AD treatment.