Genetic identification of rickettsiae isolated from ticks in Japan
Genetic identification of rickettsiae isolated from ticks in Japan
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DOI：
10.1128/jcm.40.6.2176-2181.2002
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发表时间：
2002-06-01
影响因子：
9.4
通讯作者：
Raoult, D
中科院分区：
文献类型：
--
作者：
Fournier, PE;Fujita, H;Raoult, D
在日本描述了由日本立克次体引起的日本斑点热病之后，对这种疾病的媒介的搜索导致从不同种类的蜱类中分离出几种立克次体。从6种不同种类的硬蜱中分离到63株立克次体，用聚合酶链式反应和单抗试验鉴定了6株模式菌株。我们通过扩增和测序编码16S rRNA和柠檬酸合成酶的基因来鉴定这六株菌株。经鉴定，分离自台湾血吸虫和黄血蜱的菌株均为日本血吸虫。在卵形硬蜱、全沟硬蜱和单刺硬蜱中，我们鉴定出一种与赫氏立克次体相同或密切相关的立克次体，海氏立克次体是一种迄今仅在欧洲发现的致病物种。最后，我们鉴定了一种新的致病性未知的基因--基因AT，该基因与从蓖麻硬蜱中获得的斯洛伐克基因密切相关。
Following the description in Japan of Japanese spotted fever, caused by Rickettsia japonica, a search for the vector of this disease led to the isolation of several rickettsiae from various tick species. Sixty-three rickettsial isolates were obtained from six different tick species, and six type strains were described by PCR and monoclonal antibody testing. We identified these six strains by amplification and sequencing of the genes encoding 16S rRNA and citrate synthase. We confirmed that the isolates from Dermacentor taiwanensis and Haemaphysalis flava ticks were R. japonica isolates. In Ixodes ovatus, Ixodes persulcatus, and Ixodes monospinosus, we identified a Rickettsia identical or closely related to Rickettsia helvetica, a species that is pathogenic for humans and that to date has only been found in Europe. Finally, we identified a new genotype of unknown pathogenicity, genotype AT, that was isolated from Amblyomma testudinarium ticks and that is closely related to a Slovakian genotype obtained from Ixodes ricinus ticks.