The roles of Tenascin C and Fibronectin 1 in adhesive capsulitis: a pilot gene expression study.
The roles of Tenascin C and Fibronectin 1 in adhesive capsulitis: a pilot gene expression study.
复制标题
DOI：
10.6061/clinics/2016(06)07
复制
发表时间：
2016-07
期刊：
影响因子：
--
通讯作者：
Faloppa F
中科院分区：
文献类型：
--
作者：
Cohen C;Leal MF;Belangero PS;Figueiredo EA;Smith MC;Andreoli CV;de Castro Pochini A;Cohen M;Ejnisman B;Faloppa F
我们评估了编码TGF-β1、TGF-β1受体、胶原修饰酶LOX、PLOD 1和PLOD 2以及细胞外基质蛋白COMP、FN 1、TNC和TNXB的基因在特发性粘连性囊炎患者滑膜/囊标本中的mRNA表达水平。还研究了测量的mRNA水平和临床参数之间的可能关联。我们从9名特发性粘连性关节囊炎患者中获得了盂肱关节滑膜/关节囊样本，这些患者在接受5个月的物理治疗后症状并未显示出改善。所有患者均经磁共振成像证实为粘连性关节囊炎。我们还从8例对照患者中获得了标本，这些患者接受了急性肩锁关节脱位手术，并且根据关节镜评估有盂肱关节囊改变的放射学指征。通过定量逆转录PCR分析滑膜/关节囊标本中的mRNA表达。使用B2 M和HPRT 1基因作为参考以标准化肩部组织样品中的靶基因表达。粘连性关节囊炎患者的滑膜/关节囊样本的TNC和FN 1表达显著高于对照组。此外，症状持续时间与TGFβ1受体I的表达直接相关。TNC和FN 1表达水平升高可能是囊损伤的标志。TGFβ1受体I的上调似乎依赖于症状持续时间;因此，TGFβ信号传导可能参与粘连性囊炎。因此，TNC、FN 1和TGFβ1受体I也可能通过促进囊炎症和纤维化在粘连性囊炎中发挥作用。
We evaluated mRNA expression levels of genes that encode TGF-β1; the TGF-β1 receptor; the collagen-modifying enzymes LOX, PLOD1, and PLOD2; and the extracellular matrix proteins COMP, FN1, TNC and TNXB in synovial/capsule specimens from patients with idiopathic adhesive capsulitis. Possible associations between the measured mRNA levels and clinical parameters were also investigated. We obtained glenohumeral joint synovium/capsule specimens from 9 patients with idiopathic adhesive capsulitis who had not shown improvement in symptoms after 5 months of physiotherapy. Adhesive capsulitis was confirmed in all patients by magnetic resonance imaging. We also obtained specimens from 8 control patients who had underwent surgery for acute acromioclavicular joint dislocation and who had radiological indication of glenohumeral capsule alteration based on arthroscopic evaluation. mRNA expression in the synovium/capsule specimens was analyzed by quantitative reverse transcription PCR. The B2M and HPRT1 genes were used as references to normalize target gene expression in the shoulder tissue samples. The synovium/capsule samples from the patients with adhesive capsulitis had significantly higher TNC and FN1 expression than those from the controls. Additionally, symptom duration directly correlated with expression of TGFβ1 receptor I. Elevated levels of TNC and FN1 expression may be a marker of capsule injury. Upregulation of TGFβ1 receptor I seems to be dependent on symptom duration; therefore, TGFβ signaling may be involved in adhesive capsulitis. As such, TNC, FN1 and TGFβ1 receptor I may also play roles in adhesive capsulitis by contributing to capsule inflammation and fibrosis.