RNA aptamer-based optical nanostructured sensor for highly sensitive and label-free detection of antigen-antibody reactions
RNA aptamer-based optical nanostructured sensor for highly sensitive and label-free detection of antigen-antibody reactions
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DOI：
10.1007/s00216-010-3488-z
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发表时间：
2010-04-01
影响因子：
4.3
通讯作者：
Tamiya, Eiichi
中科院分区：
文献类型：
--
作者：
Hiep, Ha Minh;Saito, Masato;Tamiya, Eiichi
基于贵金属的纳米结构光学蛋白质传感器的开发目前因其对从蛋白质组学到遗传学的各种重要生物分子的高效和同步分析而受到极大关注。在这项研究中，我们利用金纳米粒子基质的吸收光谱，使用特定的硫醇化 RNA 适体对抗原抗体反应进行无标记检测。这些合成的 RNA 适体经过优化，能够与人 IgG1 亚类的 Fc 部分结合，因为它们能够在金表面定向抗体方向。通过这些接头将抗纤维蛋白原抗体附着在表面后，我们的基于硫醇化 RNA 适体的纳米结构传感器可以轻松应用于纤维蛋白原的特异性检测，检测限为 0.1 ng/mL。这些基于纳米结构传感器的模型将为显示众多免疫传感器以及开发其他功能相似的传感器开辟一条道路，然后将其扩展到多阵列测定系统中。
Developments of optical protein sensors with nanostructure based on the noble metals have currently received great attention for their high efficiency and simultaneous analysis of various important biomolecules from proteomics to genetics. In this study, we exploited the absorbance spectra of gold-capped nanoparticles substrate for label-free detections of antigen-antibody reactions using a specific thiolated RNA aptamer. These synthesized RNA aptamers have been optimized to bind to the Fc portion of the human IgG1 subclass, due to their ability to orient antibodies direction on the gold surface. After attaching the anti-fibrinogen antibodies on the surface via these linkers, our thiolated RNA aptamer-based nanostructured sensors were easily applicable to specific detections of fibrinogen with a limit of detection of 0.1 ng/mL. These nanostructured sensor-based models will open a way to display numerous immunosensors as well as to develop other functionally similar sensors which could then be expanded into multi-arrays assay systems.