Hedgehog signaling is required for commitment but not initial induction of slow muscle precursors
Hedgehog signaling is required for commitment but not initial induction of slow muscle precursors
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DOI：
10.1016/j.ydbio.2004.07.030
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发表时间：
2004-11-01
影响因子：
2.7
通讯作者：
Westerfield, M
中科院分区：
文献类型：
--
作者：
Hirsinger, E;Stellabotte, F;Westerfield, M
在斑马鱼中。骨骼肌前体可以采用至少三种不同的命运：快肌纤维、非先锋慢肌纤维或先锋慢肌纤维。慢肌纤维从近轴细胞发育而来，并依赖于Hedgehog信号。我们分析了前体细胞何时决定其命运，以及Hedgehog影响其分化途径的沿着步骤。出乎意料的是，我们发现缺乏Hedgehog信号的胚胎仍然含有有丝分裂后的近轴细胞，这些细胞分化成快肌纤维而不是慢肌纤维。我们发现，由原肠胚形成的发病，慢和快的肌肉前体已经在空间上分离，但不致力于他们的命运，直到很久以后，在节段板时，慢前体成为独立的刺猬。相比之下，先锋和非先锋慢肌前体从原肠胚形成开始就有一个共同的谱系。我们的研究结果表明，慢肌前体的形式独立的刺猬信号，并进一步提供直接证据的多能肌肉前体人口的承诺，缓慢的命运取决于刺猬在发展的后期阶段时，有丝分裂后近轴细胞分化成慢肌纤维。(C)2004年爱思唯尔公司All rights reserved.
In zebrafish. skeletal muscle precursors can adopt at least three distinct fates: fast, non-pioneer slow, or pioneer slow muscle fibers. Slow muscle fibers develop from adaxial cells and depend on Hedgehog signaling. We analyzed when precursors become committed to their fates and the step(s) along their differentiation pathway affected by Hedgehog. Unexpectedly, we find that embryos deficient in Hedgehog signaling still contain postmitotic adaxial cells that differentiate into fast muscle fibers instead of slow. We show that by the onset of gastrulation, slow and fast muscle precursors are already spatially segregated but uncommitted to their fates until much later, in the segmental plate when slow precursors become independent of Hedgehog. In contrast, pioneer and non-pioneer slow muscle precursors share a common lineage from the onset of gastrulation. Our results demonstrate that slow muscle precursors form independently of Hedgehog signaling and further provide direct evidence for a multipotent muscle precursor population whose commitment to the slow fate depends on Hedgehog at a late stage of development when postmitotic adaxial cells differentiate into slow muscle fibers. (C) 2004 Elsevier Inc. All rights reserved.